Human PLEKHO1 ELISA Kit – Cat# MBS280925

Pleckstrin Homology Domain-Containing Family O Member 1 (PLEKHO1) ELISA Kit for Human

Name: Human PLEKHO1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS280925
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS280925

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLEKHO1 in samples. An antibody specific for PLEKHO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLEKHO1 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Pleckstrin homology domain-containing family O member 1 (PLEKHO1), ELISA Kit

Full Product Name

Human Pleckstrin homology domain-containing family O member 1 (PLEKHO1) ELISA Kit

Product Gene Name

PLEKHO1 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PLEKHO1. No significant cross-reactivity or interference between Human PLEKHO1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

42,330 Da

OMIM

AF168676 mRNA

3D Structure

ModBase 3D Structure for Q53GL0

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PLEKHO1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLEKHO1 purchase

MBS280925 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pleckstrin homology domain-containing family O member 1 (PLEKHO1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLEKHO1. The ELISA analytical biochemical technique of the MBS280925 kit is based on PLEKHO1 antibody-PLEKHO1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLEKHO1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLEKHO1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PLEKHO1. It may not necessarily be applicable to this product.

NCBI GI #

752993118

NCBI GeneID

51177

NCBI Accession #

NP_001291651.1

UniProt Primary Accession #

Q53GL0

UniProt Secondary Accession #

Q336K5; Q8IZ51; Q9NRV3; Q9UL48

UniProt Related Accession #

Q53GL0

GenBank Nucleotide #

NM_001304722.1

NCBI Official Full Name

pleckstrin homology domain-containing family O member 1 isoform b

NCBI Official Synonym Full Names

pleckstrin homology domain containing O1

NCBI Official Symbol

PLEKHO1

NCBI Official Synonym Symbols

JBP; CKIP1; OC120; CKIP-1

UniProt Protein Name

Pleckstrin homology domain-containing family O member 1

UniProt Synonym Protein Names

C-Jun-binding protein; JBP; Casein kinase 2-interacting protein 1; CK2-interacting protein 1; CKIP-1; Osteoclast maturation-associated gene 120 protein

Protein Family

Pleckstrin homology domain-containing family

UniProt Gene Name

PLEKHO1

UniProt Synonym Gene Names

CKIP1; OC120; PH domain-containing family O member 1; JBP; CK2-interacting protein 1; CKIP-1

NCBI Protein Information

pleckstrin homology domain-containing family O member 1

UniProt Comments for PLEKHO1

PLEKHO1: Plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). May function to target CK2 to the plasma membrane thereby serving as an adapter to facilitate the phosphorylation of CP by protein kinase 2 (CK2). Appears to target ATM to the plasma membrane. Appears to also inhibit tumor cell growth by inhibiting AKT- mediated cell-survival. Also implicated in PI3K-regulated muscle differentiation, the regulation of AP-1 activity (plasma membrane bound AP-1 regulator that translocates to the nucleus) and the promotion of apoptosis induced by tumor necrosis factor TNF. When bound to PKB, it inhibits it probably by decreasing PKB level of phosphorylation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Tumor suppressor

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: myoblast fusion; myoblast migration; regulation of cell shape

Research Articles on PLEKHO1

1. these data integrated CKIP-1 expression and function as a novel transcriptional regulator of macrophage polarization.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PLEKHO1 elisa kit

Reference Product	PubMed Publications
SMURF1 elisa kit	>3 publications with PLEKHO1 and SMURF1
AKT3 elisa kit	>1 publications with PLEKHO1 and AKT3
AKT2 elisa kit	>1 publications with PLEKHO1 and AKT2
AKT1 elisa kit	>1 publications with PLEKHO1 and AKT1
SMAD5 elisa kit	>1 publications with PLEKHO1 and SMAD5

Diseases associated with PLEKHO1 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>2 publications with PLEKHO1 and Inflammation
Cell Transformation, Neoplastic Antibodies	>1 publications with PLEKHO1 and Cell Transformation, Neoplastic
Necrosis Antibodies	>1 publications with PLEKHO1 and Necrosis

Organs/Tissues associated with PLEKHO1 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>8 publications with PLEKHO1 and Bone
Blood Antibodies	>6 publications with PLEKHO1 and Blood
Muscle Antibodies	>4 publications with PLEKHO1 and Muscle
Connective Tissue Antibodies	>1 publications with PLEKHO1 and Connective Tissue
Kidney Antibodies	>1 publications with PLEKHO1 and Kidney
Prostate Antibodies	>1 publications with PLEKHO1 and Prostate
Lung Antibodies	>1 publications with PLEKHO1 and Lung