Permeability Glyco (P-gp) ELISA Kit for Human

Name: Human P-gp ELISA Kit; Permeability Glyco; MyBioSource
Brand: MyBioSource
SKU: MBS281012
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281012

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ABCB1 in samples. An antibody specific for ABCB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCB1 present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Permeability glycoprotein (P-gp), ELISA Kit

Full Product Name

Human Permeability glycoprotein (P-gp) ELISA Kit

Product Gene Name

P-gp elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human ABCB1. No significant cross-reactivity or interference between Human ABCB1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

134,032 Da

OMIM

M14758 mRNA

3D Structure

ModBase 3D Structure for P08183

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of P-gp elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for P-gp purchase

MBS281012 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Permeability glycoprotein (P-gp) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing P-gp. The ELISA analytical biochemical technique of the MBS281012 kit is based on P-gp antibody-P-gp antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect P-gp antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, P-gp. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for P-gp. It may not necessarily be applicable to this product.

NCBI GI #

42741659

NCBI GeneID

5243

NCBI Accession #

NP_000918.2

UniProt Primary Accession #

P08183

UniProt Secondary Accession #

Q12755; Q14812; A8K294; B5AK60

UniProt Related Accession #

P08183

GenBank Nucleotide #

NM_000927.4

NCBI Official Full Name

multidrug resistance protein 1 isoform 2

NCBI Official Synonym Full Names

ATP binding cassette subfamily B member 1

NCBI Official Symbol

ABCB1

NCBI Official Synonym Symbols

CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170

UniProt Protein Name

Multidrug resistance protein 1

UniProt Synonym Protein Names

ATP-binding cassette sub-family B member 1; P-glycoprotein 1; CD_antigen: CD243

UniProt Gene Name

ABCB1

UniProt Synonym Gene Names

MDR1; PGY1

NCBI Protein Information

multidrug resistance protein 1

NCBI Summary for P-gp

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. Mutations in this gene are associated with colchicine resistance and Inflammatory bowel disease 13. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Feb 2017]

UniProt Comments for P-gp

ABCB1: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. Interacts with PSMB8.

Protein type: Cell adhesion; EC 3.6.3.44; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 7q21.12

Cellular Component: apical plasma membrane; cell surface; extracellular exosome; integral component of membrane; membrane; plasma membrane

Molecular Function: ATP binding; ATPase activity, coupled to transmembrane movement of substances; ceramide-translocating ATPase activity; phosphatidylcholine-translocating ATPase activity; phosphatidylethanolamine-translocating ATPase activity; protein binding; transporter activity; xenobiotic transmembrane transporting ATPase activity

Biological Process: ceramide translocation; drug transmembrane transport; G2/M transition of mitotic cell cycle; phospholipid translocation; positive regulation of anion channel activity; regulation of chloride transport; regulation of response to osmotic stress; response to drug; stem cell proliferation; transmembrane transport; transport; xenobiotic transport

Disease: Colchicine Resistance; Inflammatory Bowel Disease 13

Research Articles on P-gp

1. we review the emerging role of molecular dynamics simulation techniques in our understanding of the membrane-embedded conformation of P-gp--{REVIEW}

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with P-gp elisa kit

Reference Product	PubMed Publications
HIF1A elisa kit	>47 publications with P-gp and HIF1A
TP53 elisa kit	>33 publications with P-gp and TP53
SP1 elisa kit	>30 publications with P-gp and SP1
SLC22A1 elisa kit	>26 publications with P-gp and SLC22A1
CAV1 elisa kit	>25 publications with P-gp and CAV1
SLC22A2 elisa kit	>15 publications with P-gp and SLC22A2
NR1I3 elisa kit	>12 publications with P-gp and NR1I3
ARNT elisa kit	>4 publications with P-gp and ARNT
LANCL2 elisa kit	>1 publications with P-gp and LANCL2

Pathways associated with P-gp elisa kit

Products by Pathway	Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
ABC Transporters Pathway antibodies	ABC Transporters Pathway Diagram
ABC-family Proteins Mediated Transport Pathway antibodies	ABC-family Proteins Mediated Transport Pathway Diagram
Abacavir Transmembrane Transport Pathway antibodies	Abacavir Transmembrane Transport Pathway Diagram
Abacavir Transport And Metabolism Pathway antibodies	Abacavir Transport And Metabolism Pathway Diagram
Bile Secretion Pathway antibodies	Bile Secretion Pathway Diagram
Bile Secretion Pathway antibodies	Bile Secretion Pathway Diagram
Codeine And Morphine Metabolism Pathway antibodies	Codeine And Morphine Metabolism Pathway Diagram
Drug Induction Of Bile Acid Pathway antibodies	Drug Induction Of Bile Acid Pathway Diagram
HIF-1-alpha Transcription Factor Network Pathway antibodies	HIF-1-alpha Transcription Factor Network Pathway Diagram

Diseases associated with P-gp elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>6720 publications with P-gp and Neoplasms
Carcinoma Antibodies	>2221 publications with P-gp and Carcinoma
Drug Toxicity Antibodies	>1688 publications with P-gp and Drug Toxicity
Gastrointestinal Diseases Antibodies	>783 publications with P-gp and Gastrointestinal Diseases
Nervous System Diseases Antibodies	>748 publications with P-gp and Nervous System Diseases
Liver Diseases Antibodies	>531 publications with P-gp and Liver Diseases
Epilepsy Antibodies	>310 publications with P-gp and Epilepsy
Carcinoma, Non-Small-Cell Lung Antibodies	>304 publications with P-gp and Carcinoma, Non-Small-Cell Lung
Kidney Diseases Antibodies	>280 publications with P-gp and Kidney Diseases
Disease Progression Antibodies	>279 publications with P-gp and Disease Progression

Organs/Tissues associated with P-gp elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>2162 publications with P-gp and Brain
Placenta Antibodies	>182 publications with P-gp and Placenta
Uterus Antibodies	>27 publications with P-gp and Uterus
Trachea Antibodies	>8 publications with P-gp and Trachea