Macrophage Colony-Stimulating Factor (M-CSF) ELISA Kit for Simian

Name: Simian M-CSF ELISA Kit; Macrophage Colony-Stimulating Factor; MyBioSource
Brand: MyBioSource
SKU: MBS281081
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281081

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MCSF in samples. An antibody specific for MCSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCSF present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Macrophage Colony-Stimulating Factor (M-CSF), ELISA Kit

Full Product Name

Simian Macrophage Colony-Stimulating Factor (M-CSF) ELISA Kit

Product Gene Name

M-CSF elisa kit

Species Reactivity

Simian

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of SimianMCSF. No significant cross-reactivity or interference between SimianMCSF and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

16,295 Da

OMIM

M13207 Genomic DNA

3D Structure

ModBase 3D Structure for P04141

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of M-CSF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for M-CSF purchase

MBS281081 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Macrophage Colony-Stimulating Factor (M-CSF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing M-CSF. The ELISA analytical biochemical technique of the MBS281081 kit is based on M-CSF antibody-M-CSF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect M-CSF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, M-CSF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for M-CSF. It may not necessarily be applicable to this product.

NCBI GI #

27437030

NCBI GeneID

1437

NCBI Accession #

NP_000749.2

UniProt Primary Accession #

P04141

UniProt Secondary Accession #

Q14CE8; Q2VPI8; Q8NFI6

UniProt Related Accession #

P04141

GenBank Nucleotide #

NM_000758.3

NCBI Official Full Name

granulocyte-macrophage colony-stimulating factor

NCBI Official Synonym Full Names

colony stimulating factor 2

NCBI Official Symbol

CSF2

NCBI Official Synonym Symbols

CSF; GMCSF

UniProt Protein Name

Granulocyte-macrophage colony-stimulating factor

UniProt Synonym Protein Names

Colony-stimulating factor; CSF; Molgramostin; Sargramostim

Protein Family

Macrophage colony-stimulating factor

UniProt Gene Name

CSF2

UniProt Synonym Gene Names

GMCSF; GM-CSF; CSF

NCBI Protein Information

granulocyte-macrophage colony-stimulating factor

NCBI Summary for M-CSF

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

UniProt Comments for M-CSF

GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular region; extracellular space; intracellular membrane-bound organelle

Molecular Function: cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity; protein binding; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: embryonic placenta development; epithelial fluid transport; histamine secretion; immune response; macrophage activation; MAPK cascade; monocyte differentiation; myeloid dendritic cell differentiation; negative regulation of cytolysis; neutrophil differentiation; peptidyl-tyrosine phosphorylation; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of GTPase activity; positive regulation of interleukin-23 production; positive regulation of tyrosine phosphorylation of STAT protein; regulation of circadian sleep/wake cycle, sleep; regulation of myeloid cell differentiation; response to silicon dioxide

Research Articles on M-CSF

1. Oral and periodontal innate immunity is affected by HIV viremia and ART. GCF IL-8, G-CSF, as well as serum IL-8, MCP-1 and GM-CSF may be useful biomarkers for the detection of disease presence and/or its severity due to HIV infection and ART use.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with M-CSF elisa kit

Products by Pathway	Pathway Diagram
ARMS-mediated Activation Pathway antibodies	ARMS-mediated Activation Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Calcium Signaling In The CD4+ TCR Pathway antibodies	Calcium Signaling In The CD4+ TCR Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokines And Inflammatory Response Pathway antibodies	Cytokines And Inflammatory Response Pathway Diagram

Diseases associated with M-CSF elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>52 publications with M-CSF and Inflammation
Necrosis Antibodies	>30 publications with M-CSF and Necrosis
Lung Diseases Antibodies	>14 publications with M-CSF and Lung Diseases
Carcinoma Antibodies	>9 publications with M-CSF and Carcinoma
Breast Neoplasms Antibodies	>7 publications with M-CSF and Breast Neoplasms
Fibrosis Antibodies	>5 publications with M-CSF and Fibrosis
Neoplasms, Experimental Antibodies	>4 publications with M-CSF and Neoplasms, Experimental
Lung Neoplasms Antibodies	>4 publications with M-CSF and Lung Neoplasms
Leukemia, Myeloid, Acute Antibodies	>3 publications with M-CSF and Leukemia, Myeloid, Acute
Arthritis, Rheumatoid Antibodies	>3 publications with M-CSF and Arthritis, Rheumatoid

Organs/Tissues associated with M-CSF elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>60 publications with M-CSF and Blood
Bone Antibodies	>21 publications with M-CSF and Bone
Lung Antibodies	>18 publications with M-CSF and Lung
Intestine Antibodies	>3 publications with M-CSF and Intestine