Cardiac Phospholamban (PLN) ELISA Kit for Mouse

Name: Mouse PLN ELISA Kit; Cardiac Phospholamban; MyBioSource
Brand: MyBioSource
SKU: MBS281096
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281096

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLN in samples. An antibody specific for PLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLN present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Cardiac phospholamban (PLN), ELISA Kit

Full Product Name

Mouse Cardiac phospholamban (PLN) ELISA Kit

Product Gene Name

PLN elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse PLN. No significant cross-reactivity or interference between Mouse PLN and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

6,095 Da

3D Structure

ModBase 3D Structure for P61014

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PLN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PLN purchase

MBS281096 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cardiac phospholamban (PLN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLN. The ELISA analytical biochemical technique of the MBS281096 kit is based on PLN antibody-PLN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PLN. It may not necessarily be applicable to this product.

NCBI GI #

213512816

NCBI GeneID

18821

NCBI Accession #

NP_001135399.1

UniProt Primary Accession #

P61014

UniProt Secondary Accession #

P20006; Q3UQ75

UniProt Related Accession #

P61014

GenBank Nucleotide #

NM_001141927.1

NCBI Official Full Name

cardiac phospholamban

NCBI Official Synonym Full Names

phospholamban

NCBI Official Symbol

Pln

NCBI Official Synonym Symbols

Plb

UniProt Protein Name

Cardiac phospholamban

Protein Family

Cardiac phospholamban

UniProt Gene Name

Pln

UniProt Synonym Gene Names

PLB

NCBI Protein Information

cardiac phospholamban

UniProt Comments for PLN

PLB: a heart protein postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum. A major substrate for the cAMP-dependent protein kinase. An inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. A key regulator of cardiac diastolic function. Mutations are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

Protein type: Inhibitor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10|10 B3

Cellular Component: endoplasmic reticulum; integral component of membrane; membrane; mitochondrion; protein complex; sarcoplasmic reticulum; sarcoplasmic reticulum membrane; vesicle

Molecular Function: ATPase inhibitor activity; calcium channel regulator activity; protein binding

Biological Process: adrenergic receptor signaling pathway involved in heart process; calcium ion transport; cardiac muscle tissue development; cellular calcium ion homeostasis; negative regulation of heart contraction; Notch signaling pathway; protein homooligomerization; regulation of calcium ion import; regulation of cardiac muscle cell contraction; regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion; regulation of heart contraction; regulation of relaxation of muscle; regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum; regulation of the force of heart contraction; regulation of the force of heart contraction by cardiac conduction; response to testosterone stimulus; response to zinc ion

Research Articles on PLN

1. A single-dose injection of PLN-targeting locked nucleic acid antisense oligonucleotide improved contractility in pressure overload-induced cardiac dysfunction.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PLN elisa kit

Reference Product	PubMed Publications
Atp2a2 elisa kit	>20 publications with PLN and Atp2a2
Tnni3 elisa kit	>8 publications with PLN and Tnni3
Myh6 elisa kit	>4 publications with PLN and Myh6
Atp2a1 elisa kit	>3 publications with PLN and Atp2a1

Pathways associated with PLN elisa kit

Products by Pathway	Pathway Diagram
Adrenergic Signaling In Cardiomyocytes Pathway antibodies	Adrenergic Signaling In Cardiomyocytes Pathway Diagram
Adrenergic Signaling In Cardiomyocytes Pathway antibodies	Adrenergic Signaling In Cardiomyocytes Pathway Diagram
Calcium Regulation In The Cardiac Cell Pathway antibodies	Calcium Regulation In The Cardiac Cell Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Cardiac Conduction Pathway antibodies	Cardiac Conduction Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Dilated Cardiomyopathy Pathway antibodies	Dilated Cardiomyopathy Pathway Diagram
Ion Channel Transport Pathway antibodies	Ion Channel Transport Pathway Diagram
Ion Homeostasis Pathway antibodies	Ion Homeostasis Pathway Diagram

Diseases associated with PLN elisa kit

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>139 publications with PLN and Heart Diseases
Heart Failure Antibodies	>88 publications with PLN and Heart Failure
Disease Models, Animal Antibodies	>73 publications with PLN and Disease Models, Animal
Inflammation Antibodies	>71 publications with PLN and Inflammation
Adenocarcinoma Antibodies	>46 publications with PLN and Adenocarcinoma
Kidney Diseases Antibodies	>40 publications with PLN and Kidney Diseases
Hypertrophy Antibodies	>39 publications with PLN and Hypertrophy
Vascular Diseases Antibodies	>38 publications with PLN and Vascular Diseases
Cardiomegaly Antibodies	>37 publications with PLN and Cardiomegaly
Uterine Cervical Neoplasms Antibodies	>35 publications with PLN and Uterine Cervical Neoplasms

Organs/Tissues associated with PLN elisa kit

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>261 publications with PLN and Heart
Muscle Antibodies	>250 publications with PLN and Muscle
Other Antibodies	>211 publications with PLN and Other
Kidney Antibodies	>48 publications with PLN and Kidney
Thymus Antibodies	>32 publications with PLN and Thymus
Embryonic Tissue Antibodies	>16 publications with PLN and Embryonic Tissue
Uncharacterized Tissue Antibodies	>1 publications with PLN and Uncharacterized Tissue