Plasma Kallikrein (KLKB1) ELISA Kit for Human

Name: Human KLKB1 ELISA Kit; Plasma Kallikrein; MyBioSource
Brand: MyBioSource
SKU: MBS281175
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281175

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KLKB1 in samples. An antibody specific for KLKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKLKB1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Plasma kallikrein (KLKB1), ELISA Kit

Full Product Name

Human Plasma kallikrein (KLKB1) ELISA Kit

Product Gene Name

KLKB1 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human KLKB1. No significant cross-reactivity or interference between Human KLKB1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

71,383 Da

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of KLKB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KLKB1 purchase

MBS281175 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Plasma kallikrein (KLKB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KLKB1. The ELISA analytical biochemical technique of the MBS281175 kit is based on KLKB1 antibody-KLKB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KLKB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KLKB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for KLKB1. It may not necessarily be applicable to this product.

NCBI GI #

236465805

NCBI GeneID

16621

NCBI Accession #

NP_032481.2

UniProt Secondary Accession #

Q8R0P5

UniProt Related Accession #

P26262

GenBank Nucleotide #

NM_008455.2

NCBI Official Full Name

plasma kallikrein

NCBI Official Synonym Full Names

kallikrein B, plasma 1

NCBI Official Symbol

Klkb1

NCBI Official Synonym Symbols

APS; PSA; Kal3; Klk3; Kal-3

UniProt Protein Name

Plasma kallikrein

UniProt Synonym Protein Names

Fletcher factor; Kininogenin; Plasma prekallikreinCleaved into the following 2 chains:Plasma kallikrein heavy chain; Plasma kallikrein light chain

Protein Family

Plasma kallikrein

UniProt Gene Name

Klkb1

UniProt Synonym Gene Names

Klk3; Pk

UniProt Entry Name

KLKB1_MOUSE

NCBI Protein Information

plasma kallikrein; kininogenin; fletcher factor; kallikrein 3, plasma; plasma prekallikrein; antigen, prostate specific

UniProt Comments for KLKB1

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily.

Protein type: EC 3.4.21.34; Protease; Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: fibrinolysis; hemostasis; positive regulation of fibrinolysis; proteolysis; inflammatory response; blood coagulation; plasminogen activation

Research Articles on KLKB1

1. Plasma kallikrein deletion prevents occlusive thrombus formation in mice with a minimal role in provoked bleeding.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KLKB1 elisa kit

Reference Product	PubMed Publications
F11 elisa kit	>8 publications with KLKB1 and F11
F12 elisa kit	>4 publications with KLKB1 and F12
Kng1 elisa kit	>2 publications with KLKB1 and Kng1

Pathways associated with KLKB1 elisa kit

Products by Pathway	Pathway Diagram
Activation Of Matrix Metalloproteinases Pathway antibodies	Activation Of Matrix Metalloproteinases Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Complement And Coagulation Cascades Pathway antibodies	Complement And Coagulation Cascades Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Intrinsic Pathway antibodies	Intrinsic Pathway Diagram

Diseases associated with KLKB1 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>9 publications with KLKB1 and Cardiovascular Diseases
Hypertension Antibodies	>4 publications with KLKB1 and Hypertension
Kidney Diseases Antibodies	>2 publications with KLKB1 and Kidney Diseases
Prostatic Diseases Antibodies	>1 publications with KLKB1 and Prostatic Diseases
Brain Diseases Antibodies	>1 publications with KLKB1 and Brain Diseases
Congenital Abnormalities Antibodies	>1 publications with KLKB1 and Congenital Abnormalities
Mental Disorders Antibodies	>1 publications with KLKB1 and Mental Disorders
Inflammation Antibodies	>1 publications with KLKB1 and Inflammation
Liver Diseases Antibodies	>1 publications with KLKB1 and Liver Diseases
Prekallikrein Deficiency Antibodies	>1 publications with KLKB1 and Prekallikrein Deficiency

Organs/Tissues associated with KLKB1 elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>4 publications with KLKB1 and Kidney
Liver Antibodies	>2 publications with KLKB1 and Liver