Piggybac Transposable Element-Derived Protein 3 (PGBD3) ELISA Kit for Human

Name: Human PGBD3 ELISA Kit; Piggybac Transposable Element-Derived Protein 3; MyBioSource
Brand: MyBioSource
SKU: MBS281255
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281255

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PGBD3 in samples. An antibody specific for PGBD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGBD3 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

PiggyBac transposable element-derived protein 3 (PGBD3), ELISA Kit

Full Product Name

Human PiggyBac transposable element-derived protein 3 (PGBD3) ELISA Kit

Product Gene Name

PGBD3 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PGBD3. No significant cross-reactivity or interference between Human PGBD3 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

119,487 Da

OMIM

AK074682 mRNA

3D Structure

ModBase 3D Structure for Q8N328

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PGBD3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGBD3 purchase

MBS281255 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PiggyBac transposable element-derived protein 3 (PGBD3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PGBD3. The ELISA analytical biochemical technique of the MBS281255 kit is based on PGBD3 antibody-PGBD3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PGBD3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PGBD3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PGBD3. It may not necessarily be applicable to this product.

NCBI GI #

284055225

NCBI GeneID

267004

NCBI Accession #

NP_736609.2

UniProt Primary Accession #

Q8N328

UniProt Secondary Accession #

Q5W0M0; Q6PIH0; B3KQC4

UniProt Related Accession #

Q8N328

GenBank Nucleotide #

NM_170753.3

NCBI Official Full Name

piggyBac transposable element-derived protein 3

NCBI Official Synonym Full Names

piggyBac transposable element derived 3

NCBI Official Symbol

PGBD3

UniProt Protein Name

PiggyBac transposable element-derived protein 3

Protein Family

PiggyBac transposable element-derived protein

UniProt Gene Name

PGBD3

NCBI Protein Information

piggyBac transposable element-derived protein 3

NCBI Summary for PGBD3

This gene is a member of a small family of genes derived from piggyBac transposable elements. The encoded protein contains a zinc-ribbon domain characteristic of transposon-derived proteins and may function as a regulator of transcription. Alternative splicing occurs between a splice site from exon 5 of the adjacent upstream gene 'excision repair cross-complementation group 6' (ERCC6, GeneID: 2074) and the 3' splice site upstream of the open reading frame (ORF) of this gene, which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene. Pseudogenes for this gene are defined on chromosomes 4, 5 and 12. [provided by RefSeq, Mar 2016]

UniProt Comments for PGBD3

PGBD3:

Chromosomal Location of Human Ortholog: 10q11.23

Cellular Component: nucleus

Molecular Function: DNA binding

Research Articles on PGBD3

1. CSB-PGBD3 fusion protein is important in both health and disease, and could play a role in Cockayne syndrome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PGBD3 elisa kit

Products by Pathway	Pathway Diagram
DNA Repair Pathway antibodies	DNA Repair Pathway Diagram
Dual Incision Reaction In TC-NER Pathway antibodies	Dual Incision Reaction In TC-NER Pathway Diagram
Formation Of Transcription-coupled NER (TC-NER) Repair Complex Pathway antibodies	Formation Of Transcription-coupled NER (TC-NER) Repair Complex Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Nucleotide Excision Repair Pathway antibodies	Nucleotide Excision Repair Pathway Diagram
RNA Polymerase I Promoter Clearance Pathway antibodies	RNA Polymerase I Promoter Clearance Pathway Diagram
RNA Polymerase I Transcription Pathway antibodies	RNA Polymerase I Transcription Pathway Diagram
RNA Polymerase I Transcription Initiation Pathway antibodies	RNA Polymerase I Transcription Initiation Pathway Diagram
RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies	RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
Transcription-coupled NER (TC-NER) Pathway antibodies	Transcription-coupled NER (TC-NER) Pathway Diagram

Organs/Tissues associated with PGBD3 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>1 publications with PGBD3 and Brain