Zinc Finger CCHC Domain-Containing Protein 12 (ZCCHC12) ELISA Kit for Mouse

The 402-amino acid Zcchc12 protein contains an N-terminal paraneoplastic antigen (MA)-like region, a C2HC-type zinc finger motif, and a putative nuclear localization signal. Northern blot analysis detected restricted expression of Zcchc12 in the ventral forebrain (including septum, amygdala, putamen, thalamus, and hypothalamus) of E11.5-E12.5 mice, and predominant expression in the brain and lower expression in the testis of adult mice.
Zcchc12 protein binds directly to Smad1. Zcchc12 functions as a transcriptional coactivator necessary for bone morphogenetic protein (BMP)-dependent, cholinergic-neuron-specific gene expression.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZCCHC12 in samples. An antibody specific for ZCCHC12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZCCHC12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZCCHC12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZCCHC12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of ZCCHC12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281268 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Zinc finger CCHC domain-containing protein 12 (ZCCHC12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZCCHC12. The ELISA analytical biochemical technique of the MBS281268 kit is based on ZCCHC12 antibody-ZCCHC12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZCCHC12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZCCHC12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).