• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Carbonic Anhydrase 2 (CA2) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281372

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CA2 in samples. An antibody specific for CA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCA2 present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Carbonic anhydrase 2 (CA2), ELISA Kit
Full Product Name
Mouse Carbonic anhydrase 2 (CA2) ELISA Kit
Product Gene Name
CA2 elisa kit
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CA2. No significant cross-reactivity or interference between Mouse CA2 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
29,033 Da
3D Structure
ModBase 3D Structure for P00920
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CA2 purchase

MBS281372 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Carbonic anhydrase 2 (CA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CA2. The ELISA analytical biochemical technique of the MBS281372 kit is based on CA2 antibody-CA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CA2. It may not necessarily be applicable to this product.

NCBI GI #
157951596
NCBI GeneID
12349
NCBI Accession #
NP_033931.4
UniProt Primary Accession #
P00920
UniProt Secondary Accession #
Q6LDQ7; Q7TPE1; Q9DCT3; Q9DCY9
UniProt Related Accession #
P00920
GenBank Nucleotide #
NM_009801.5
NCBI Official Full Name
carbonic anhydrase 2
NCBI Official Synonym Full Names
carbonic anhydrase 2
NCBI Official Symbol
Car2
NCBI Official Synonym Symbols
Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712
UniProt Protein Name
Carbonic anhydrase 2
UniProt Synonym Protein Names
Carbonate dehydratase II; Carbonic anhydrase II; CA-II
Protein Family
Beta-carotene hydroxylase
UniProt Gene Name
Ca2
UniProt Synonym Gene Names
Car2; CA-II
NCBI Protein Information
carbonic anhydrase 2

UniProt Comments for CA2

CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase

Chromosomal Location of Human Ortholog: 3 A1|3 3.23 cM

Cellular Component: apical part of cell; axon; basolateral plasma membrane; cytoplasm; cytosol; extracellular exosome; extracellular space; membrane; microvillus; myelin sheath; plasma membrane

Molecular Function: arylesterase activity; carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding

Biological Process: angiotensin-activated signaling pathway; carbon dioxide transport; morphogenesis of an epithelium; one-carbon metabolic process; positive regulation of bone resorption; positive regulation of cellular pH reduction; positive regulation of dipeptide transmembrane transport; positive regulation of osteoclast differentiation; positive regulation of synaptic transmission, GABAergic; regulation of chloride transport; regulation of intracellular pH; regulation of pH; secretion
Show more

Research Articles on CA2

1. It was concluded that both MCT1 and CAII are involved in the homeostatic control of pH in skeletal muscle, both at rest and at the onset of exercise. The improved muscle function and resistance to fatigue in MCT1(+/-) mice remain unexplained.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CA2 elisa kit

 Reference Product  PubMed Publications
 SLC9A1 elisa kit  >26 publications with CA2 and SLC9A1
 SLC4A4 elisa kit  >5 publications with CA2 and SLC4A4
 SLC4A1 elisa kit  >3 publications with CA2 and SLC4A1
 SLC4A3 elisa kit  >2 publications with CA2 and SLC4A3
 SLC4A2 elisa kit  >1 publications with CA2 and SLC4A2
 DYNLL1 elisa kit  >1 publications with CA2 and DYNLL1

Pathways associated with CA2 elisa kit

 Products by Pathway  Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Bile Secretion Pathway antibodies  Bile Secretion Pathway Diagram
 Collecting Duct Acid Secretion Pathway antibodies  Collecting Duct Acid Secretion Pathway Diagram
 Collecting Duct Acid Secretion Pathway antibodies  Collecting Duct Acid Secretion Pathway Diagram
 Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway antibodies  Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway Diagram
 Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway antibodies  Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Gastric Acid Secretion Pathway antibodies  Gastric Acid Secretion Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Nitrogen Metabolism Pathway antibodies  Nitrogen Metabolism Pathway Diagram

Diseases associated with CA2 elisa kit

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >3 publications with CA2 and Cardiovascular Diseases
 Inflammation Antibodies  >3 publications with CA2 and Inflammation
 Necrosis Antibodies  >3 publications with CA2 and Necrosis
 Nervous System Diseases Antibodies  >2 publications with CA2 and Nervous System Diseases
 Disease Models, Animal Antibodies  >2 publications with CA2 and Disease Models, Animal
 Seizures Antibodies  >1 publications with CA2 and Seizures
 Edema Antibodies  >1 publications with CA2 and Edema
 Fatty Liver Antibodies  >1 publications with CA2 and Fatty Liver
 Hypertrophy Antibodies  >1 publications with CA2 and Hypertrophy
 Hyperplasia Antibodies  >1 publications with CA2 and Hyperplasia

Organs/Tissues associated with CA2 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >18850 publications with CA2 and Brain
 Blood Antibodies  >16731 publications with CA2 and Blood
 Heart Antibodies  >13693 publications with CA2 and Heart
 Liver Antibodies  >4765 publications with CA2 and Liver
 Kidney Antibodies  >4271 publications with CA2 and Kidney
 Pancreas Antibodies  >3166 publications with CA2 and Pancreas
 Intestine Antibodies  >2432 publications with CA2 and Intestine
 Eye Antibodies  >2382 publications with CA2 and Eye
 Lung Antibodies  >1829 publications with CA2 and Lung
 Embryonic Tissue Antibodies  >1677 publications with CA2 and Embryonic Tissue
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000