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Casein Kinase 1 Isoform Delta (CSNK1D) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281420

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CSNK1D in samples. An antibody specific for CSNK1D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCSNK1D present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Casein kinase I isoform delta (CSNK1D), ELISA Kit
Full Product Name
Mouse Casein kinase I isoform delta (CSNK1D) ELISA Kit
Product Gene Name
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CSNK1D. No significant cross-reactivity or interference between Mouse CSNK1D and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
46,818 Da
3D Structure
ModBase 3D Structure for Q9DC28
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CSNK1D elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CSNK1D purchase

MBS281420 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Casein kinase I isoform delta (CSNK1D) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CSNK1D. The ELISA analytical biochemical technique of the MBS281420 kit is based on CSNK1D antibody-CSNK1D antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CSNK1D antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CSNK1D. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CSNK1D. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
casein kinase I isoform delta isoform 1
NCBI Official Synonym Full Names
casein kinase 1, delta
NCBI Official Symbol
Csnk1d
NCBI Official Synonym Symbols
AA409348; 1200006A05Rik; D930010H05Rik
UniProt Protein Name
Casein kinase I isoform delta
UniProt Synonym Protein Names
Tau-protein kinase CSNK1D (EC:2.7.11.26)
Protein Family
UniProt Gene Name
Csnk1d
UniProt Synonym Gene Names
Hckid; CKI-delta; CKId
NCBI Protein Information
casein kinase I isoform delta; casein kinase I

NCBI Summary for CSNK1D

This gene encodes a member of the casein kinase I (CKI) family of serine/threonine protein kinases. A highly similar human protein regulates an array of cellular processes by influencing the Wnt and hedgehog signaling pathways. The encoded protein may also be involved in the regulation of apoptosis, circadian rhythm, microtubule dynamics, chromosome segregation, and p53-mediated effects on growth. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for CSNK1D

CK1D: an ubiquitous protein kinase of the CK1 family. Enriched at the centrosomes in interphase and at the spindle during mitosis. May play a role in cell cycle progression. Two splice variant isoforms have been described.

Protein type: CK1 group; CK1 family; EC 2.7.11.1; EC 2.7.11.26; Kinase, protein; Protein kinase, CK1; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 11|11 E2

Cellular Component: centrosome; cytoplasm; cytoskeleton; Golgi apparatus; membrane; neuron projection; nucleus; perinuclear region of cytoplasm; plasma membrane; spindle; spindle microtubule

Molecular Function: ATP binding; cadherin binding; kinase activity; nucleotide binding; peptide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; tau-protein kinase activity; transferase activity

Biological Process: circadian regulation of gene expression; endocytosis; Golgi organization and biogenesis; microtubule nucleation; non-motile cilium assembly; peptidyl-serine phosphorylation; phosphorylation; positive regulation of canonical Wnt signaling pathway; positive regulation of non-canonical Wnt signaling pathway; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein phosphorylation; positive regulation of Wnt receptor signaling pathway; positive regulation of Wnt-mediated midbrain dopaminergic neuron differentiation; protein amino acid phosphorylation; protein localization to centrosome; protein localization to Golgi apparatus; regulation of cell shape; regulation of circadian rhythm; rhythmic process; spindle assembly; Wnt signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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