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Carbohydrate Sulfotransferase 14 (CHST14) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281479

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CHST14 in samples. An antibody specific for CHST14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHST14 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Carbohydrate sulfotransferase 14 (CHST14), ELISA Kit
Full Product Name
Mouse Carbohydrate sulfotransferase 14 (CHST14) ELISA Kit
Product Gene Name
CHST14 elisa kit
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CHST14. No significant cross-reactivity or interference between Mouse CHST14 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
42,997 Da
OMIM
AF401222 mRNA
3D Structure
ModBase 3D Structure for Q8NCH0
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CHST14 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHST14 purchase

MBS281479 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Carbohydrate sulfotransferase 14 (CHST14) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHST14. The ELISA analytical biochemical technique of the MBS281479 kit is based on CHST14 antibody-CHST14 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHST14 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHST14. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CHST14. It may not necessarily be applicable to this product.

NCBI GI #
18497304
NCBI GeneID
113189
NCBI Accession #
NP_569735.1
UniProt Primary Accession #
Q8NCH0
UniProt Secondary Accession #
Q6PJ31; Q6UXA0; Q96P94
UniProt Related Accession #
Q8NCH0
GenBank Nucleotide #
NM_130468.3
NCBI Official Full Name
carbohydrate sulfotransferase 14
NCBI Official Synonym Full Names
carbohydrate sulfotransferase 14
NCBI Official Symbol
CHST14
NCBI Official Synonym Symbols
ATCS; D4ST1; EDSMC1; HNK1ST
UniProt Protein Name
Carbohydrate sulfotransferase 14
UniProt Synonym Protein Names
Dermatan 4-sulfotransferase 1; D4ST-1; hD4ST1
Protein Family
Carbohydrate sulfotransferase
UniProt Gene Name
CHST14
UniProt Synonym Gene Names
D4ST1; D4ST-1; hD4ST1
NCBI Protein Information
carbohydrate sulfotransferase 14

NCBI Summary for CHST14

This gene encodes a member of the HNK-1 family of sulfotransferases. The encoded protein transfers sulfate to the C-4 hydroxyl of N-acetylgalactosamine residues in dermatan sulfate. Mutations in this gene have been associated with adducted thumb-clubfoot syndrome.[provided by RefSeq, Mar 2010]

UniProt Comments for CHST14

CHST14: Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of dermatan sulfate. Plays a pivotal role in the formation of 4-0-sulfated IdoA blocks in dermatan sulfate. Transfers sulfate to the C-4 hydroxyl of beta1,4-linked GalNAc that is substituted with an alpha-linked iduronic acid (IdoUA) at the C-3 hydroxyl. Transfers sulfate more efficiently to GalNAc residues in -IdoUA-GalNAc-IdoUA- than in -GlcUA-GalNAc-GlcUA-sequences. Has preference for partially desulfated dermatan sulfate. Addition of sulfate to GalNAc may occur immediately after epimerization of GlcUA to IdoUA. GlcUA to IdoUA. Appears to have an important role in the formation of the cerbellar neural network during postnatal brain development. Defects in CHST14 are the cause of Ehlers-Danlos syndrome musculocontractural type (EDSMC). It is a form of Ehlers-Danlos syndrome characterized by distinctive craniofacial dysmorphism, congenital contractures of thumbs and fingers, clubfeet, severe kyphoscoliosis, muscular hypotonia, hyperextensible thin skin with easy bruisability and atrophic scarring, wrinkled palms, joint hypermobility, and ocular involvement. Belongs to the sulfotransferase 2 family.

Protein type: EC 2.8.2.35; Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 15q15.1

Cellular Component: Golgi membrane; integral component of membrane

Molecular Function: N-acetylgalactosamine 4-O-sulfotransferase activity; phosphate binding

Biological Process: carbohydrate biosynthetic process; dermatan sulfate biosynthetic process; dermatan sulfate proteoglycan metabolic process

Disease: Ehlers-danlos Syndrome, Musculocontractural Type 1
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Research Articles on CHST14

1. Dermatan sulfate does not appear in urine of patients with Ehlers-Danlos syndrome caused by a CHST14/D4ST1 deficiency.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CHST14 elisa kit

 Reference Product  PubMed Publications
 DCN elisa kit  >1 publications with CHST14 and DCN
 DSE elisa kit  >1 publications with CHST14 and DSE

Pathways associated with CHST14 elisa kit

 Products by Pathway  Pathway Diagram
 Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies  Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
 Dermatan Sulfate Biosynthesis Pathway antibodies  Dermatan Sulfate Biosynthesis Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway Diagram
 Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway antibodies  Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway Diagram
 Glycosaminoglycan Metabolism Pathway antibodies  Glycosaminoglycan Metabolism Pathway Diagram
 MPS I - Hurler Syndrome Pathway antibodies  MPS I - Hurler Syndrome Pathway Diagram
 MPS II - Hunter Syndrome Pathway antibodies  MPS II - Hunter Syndrome Pathway Diagram
 MPS IIIA - Sanfilippo Syndrome A Pathway antibodies  MPS IIIA - Sanfilippo Syndrome A Pathway Diagram
 MPS IIIB - Sanfilippo Syndrome B Pathway antibodies  MPS IIIB - Sanfilippo Syndrome B Pathway Diagram

Diseases associated with CHST14 elisa kit

 Disease Name  Pubmed Publications
 Craniofacial Abnormalities Antibodies  >5 publications with CHST14 and Craniofacial Abnormalities
 EHLERS-DANLOS SYNDROME, MUSCULOCONTRACTURAL TYPE Antibodies  >4 publications with CHST14 and EHLERS-DANLOS SYNDROME, MUSCULOCONTRACTURAL TYPE
 Muscular Diseases Antibodies  >3 publications with CHST14 and Muscular Diseases
 Joint Instability Antibodies  >1 publications with CHST14 and Joint Instability

Organs/Tissues associated with CHST14 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >7 publications with CHST14 and Skin
 Connective Tissue Antibodies  >4 publications with CHST14 and Connective Tissue
 Bone Antibodies  >2 publications with CHST14 and Bone
 Muscle Antibodies  >2 publications with CHST14 and Muscle
 Brain Antibodies  >2 publications with CHST14 and Brain
 Blood Antibodies  >1 publications with CHST14 and Blood
 Heart Antibodies  >1 publications with CHST14 and Heart
 Kidney Antibodies  >1 publications with CHST14 and Kidney
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