Phosphofructokinase-Platelet (PFKP) ELISA Kit for Human

Name: Human PFKP ELISA Kit; Phosphofructokinase-Platelet; MyBioSource
Brand: MyBioSource
SKU: MBS281571
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281571

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PFKP in samples. An antibody specific for PFKP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKP present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Phosphofructokinase-platelet (PFKP), ELISA Kit

Full Product Name

Human Phosphofructokinase-platelet (PFKP) ELISA Kit

Product Gene Name

PFKP elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PFKP. No significant cross-reactivity or interference between Human PFKP and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

85,316 Da

OMIM

D25328 mRNA

3D Structure

ModBase 3D Structure for Q01813

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PFKP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PFKP purchase

MBS281571 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphofructokinase-platelet (PFKP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PFKP. The ELISA analytical biochemical technique of the MBS281571 kit is based on PFKP antibody-PFKP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PFKP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PFKP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PFKP. It may not necessarily be applicable to this product.

NCBI GI #

334191701

NCBI GeneID

5214

NCBI Accession #

NP_001229268.1

UniProt Primary Accession #

Q01813

UniProt Secondary Accession #

Q5VSR7; Q5VSR8; B3KS15

UniProt Related Accession #

Q01813

GenBank Nucleotide #

NM_001242339.1

NCBI Official Full Name

ATP-dependent 6-phosphofructokinase, platelet type isoform 2

NCBI Official Synonym Full Names

phosphofructokinase, platelet

NCBI Official Symbol

PFKP

NCBI Official Synonym Symbols

PFKF; PFK-C; PFK-P; ATP-PFK

UniProt Protein Name

ATP-dependent 6-phosphofructokinase, platelet type

UniProt Synonym Protein Names

6-phosphofructokinase type C; Phosphofructo-1-kinase isozyme C; PFK-C; Phosphohexokinase

Protein Family

ATP-dependent 6-phosphofructokinase

UniProt Gene Name

PFKP

UniProt Synonym Gene Names

PFKF; ATP-PFK; PFK-P

NCBI Protein Information

ATP-dependent 6-phosphofructokinase, platelet type

NCBI Summary for PFKP

This gene encodes a member of the phosphofructokinase A protein family. The encoded enzyme is the platelet-specific isoform of phosphofructokinase and plays a key role in glycolysis regulation. This gene may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinomas, and cancer of the bladder, breast, and lung. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

UniProt Comments for PFKP

PFKP: phosphofructokinase, platelet type. An ubiquitous metabolic enzyme involved in the synthesis and degradation of fructose 2,6-bisphosphate. Key control step of glycolysis. An allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. Activity: ATP D-fructose 6-phosphate = ADP D-fructose 1,6-bisphosphate. The holoenzyme consists of 4 subunits. The liver and muscle enzymes are homo-tetramers of four liver or muscle isoforms, respectively. The red blood cell enzyme consists hetero-tetramers of the muscle and liver isoforms. A subunit composition with a higher proportion of platelet type subunits is found in platelets, brain and fibroblasts.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; EC 2.7.1.11; Kinase, other

Chromosomal Location of Human Ortholog: 10p15.2

Cellular Component: cytoplasm; cytosol; extracellular exosome; extracellular matrix; membrane; nucleus

Molecular Function: 6-phosphofructokinase activity; ATP binding; cadherin binding; metal ion binding; protein complex binding

Biological Process: fructose 6-phosphate metabolic process

Research Articles on PFKP

1. Data indicate that platelet isoform of phosphofructokinase (PFKP) not only is required for metabolic reprogramming and maintaining cell proliferation, but also may be a valid target for anti-renal cancer pharmaceutical agents.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PFKP elisa kit

Products by Pathway	Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Fructose And Mannose Metabolism Pathway antibodies	Fructose And Mannose Metabolism Pathway Diagram
Galactose Metabolism Pathway antibodies	Galactose Metabolism Pathway Diagram
Galactose Metabolism Pathway antibodies	Galactose Metabolism Pathway Diagram
Glucose Metabolism Pathway antibodies	Glucose Metabolism Pathway Diagram
Glycolysis Pathway antibodies	Glycolysis Pathway Diagram
Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies	Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies	Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram

Diseases associated with PFKP elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>3 publications with PFKP and Neoplasms
Disease Models, Animal Antibodies	>2 publications with PFKP and Disease Models, Animal
Neoplasms, Experimental Antibodies	>1 publications with PFKP and Neoplasms, Experimental
Hypertension Antibodies	>1 publications with PFKP and Hypertension
Breast Neoplasms Antibodies	>1 publications with PFKP and Breast Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with PFKP and Carcinoma, Hepatocellular

Organs/Tissues associated with PFKP elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5 publications with PFKP and Blood
Muscle Antibodies	>4 publications with PFKP and Muscle
Ovary Antibodies	>4 publications with PFKP and Ovary
Liver Antibodies	>3 publications with PFKP and Liver
Bone Antibodies	>2 publications with PFKP and Bone
Thyroid Antibodies	>2 publications with PFKP and Thyroid
Brain Antibodies	>2 publications with PFKP and Brain
Heart Antibodies	>1 publications with PFKP and Heart
Skin Antibodies	>1 publications with PFKP and Skin