Potassium Channel Subfamily K Member 10 (KCNK10) ELISA Kit for Human

KCNK10 belongs to the class of mechanosensitive and fatty acid-stimulated K+ channels.It shares 63% sequence identity with TREK (KCNK2) and 50 to 60% homology with other members of this potassium channel family. KCNK10 produces rapidly activating and noninactivating outward rectifier K+ channel currents, which are strongly stimulated by polyunsaturated fatty acids such as acrachidonic acids. KCNK10 activity is also stimulated by stretch of the cell membrane, acidification of the intracellular medium, and application of inhalational general anesthetics, and is transiently activated by neuroprotective agents such as riluzole. Since KCNK10 channel activity can be regulated by a variety of neurotransmitter receptors, the authors suggested that it plays a role as a target of neurotransmitter action.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate KCNK10 in samples. An antibody specific for KCNK10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKCNK10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCNK10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNK10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of KCNK10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281593 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Potassium channel subfamily K member 10 (KCNK10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KCNK10. The ELISA analytical biochemical technique of the MBS281593 kit is based on KCNK10 antibody-KCNK10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KCNK10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KCNK10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).