Polycystic Kidney Disease and Receptor for Egg Jelly-Related (PKDREJ) ELISA Kit for Human

Intended Uses: For the quantitative detection of Human Polycystic kidney disease and receptor for egg jelly-related protein (PKDREJ) concentration in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PKDREJ and Horseradish Peroxidase (HRP) conjugated PKDREJ. The competitive inhibition reaction is launched between with HRP labeled PKDREJ and unlabeled PKDREJ with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PKDREJ in the sample. The color development is stopped and the intensity of the color is measured.

Small volumes of PKDREJ elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281636 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Polycystic kidney disease and receptor for egg jelly-related protein (PKDREJ) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PKDREJ. The ELISA analytical biochemical technique of the MBS281636 kit is based on PKDREJ antibody-PKDREJ antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PKDREJ antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PKDREJ. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).