Carbohydrate Sulfotransferase 7 (CHST7) ELISA Kit for Mouse

Name: Mouse CHST7 ELISA Kit; Carbohydrate Sulfotransferase 7; MyBioSource
Brand: MyBioSource
SKU: MBS281729
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281729

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CHST7 in samples. An antibody specific for CHST7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHST7 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Carbohydrate sulfotransferase 7 (CHST7), ELISA Kit

Full Product Name

Mouse Carbohydrate sulfotransferase 7 (CHST7) ELISA Kit

Product Gene Name

CHST7 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse CHST7. No significant cross-reactivity or interference between Mouse CHST7 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

54,767 Da

3D Structure

ModBase 3D Structure for Q9EP78

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CHST7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHST7 purchase

MBS281729 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Carbohydrate sulfotransferase 7 (CHST7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CHST7. The ELISA analytical biochemical technique of the MBS281729 kit is based on CHST7 antibody-CHST7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CHST7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CHST7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CHST7. It may not necessarily be applicable to this product.

NCBI GI #

11096336

NCBI GeneID

60322

NCBI Accession #

NP_068361.1

UniProt Primary Accession #

Q9EP78

UniProt Secondary Accession #

Q99NB0

UniProt Related Accession #

Q9EP78

GenBank Nucleotide #

NM_021715.1

NCBI Official Full Name

carbohydrate sulfotransferase 7

NCBI Official Synonym Full Names

carbohydrate (N-acetylglucosamino) sulfotransferase 7

NCBI Official Symbol

Chst7

NCBI Official Synonym Symbols

GST5; C6ST-2; Gn6st-4; glcNAc6ST-4; 2600013M07Rik

UniProt Protein Name

Carbohydrate sulfotransferase 7

UniProt Synonym Protein Names

Chondroitin 6-sulfotransferase 2; C6ST-2; mC6ST-2; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5; GST-5; N-acetylglucosamine 6-O-sulfotransferase 4; GlcNAc6ST-4; Gn6st-4

Protein Family

Carbohydrate sulfotransferase

UniProt Gene Name

Chst7

UniProt Synonym Gene Names

Gst5; C6ST-2; mC6ST-2; GST-5; GlcNAc6ST-4; Gn6st-4

NCBI Protein Information

carbohydrate sulfotransferase 7

UniProt Comments for CHST7

CHST7: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N- acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc. Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc. Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Protein type: EC 2.8.2.17; Glycan Metabolism - chondroitin sulfate biosynthesis; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: X|X A1.3

Cellular Component: Golgi apparatus; Golgi membrane; integral component of membrane; membrane

Molecular Function: chondroitin 6-sulfotransferase activity; N-acetylglucosamine 6-O-sulfotransferase activity; sulfotransferase activity; transferase activity

Biological Process: carbohydrate metabolic process; chondroitin sulfate biosynthetic process; N-acetylglucosamine metabolic process; sulfur compound metabolic process

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CHST7 elisa kit

Products by Pathway	Pathway Diagram
Chondroitin Sulfate Biosynthesis Pathway antibodies	Chondroitin Sulfate Biosynthesis Pathway Diagram
Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway antibodies	Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Glycogen Storage Diseases Pathway antibodies	Glycogen Storage Diseases Pathway Diagram
Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway antibodies	Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway Diagram
Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway antibodies	Glycosaminoglycan Biosynthesis - Chondroitin Sulfate / Dermatan Sulfate Pathway Diagram
Glycosaminoglycan Metabolism Pathway antibodies	Glycosaminoglycan Metabolism Pathway Diagram
MPS I - Hurler Syndrome Pathway antibodies	MPS I - Hurler Syndrome Pathway Diagram
MPS II - Hunter Syndrome Pathway antibodies	MPS II - Hunter Syndrome Pathway Diagram
MPS IIIA - Sanfilippo Syndrome A Pathway antibodies	MPS IIIA - Sanfilippo Syndrome A Pathway Diagram

Diseases associated with CHST7 elisa kit

Disease Name	Pubmed Publications
Intestinal Neoplasms Antibodies	>2 publications with CHST7 and Intestinal Neoplasms
Inflammation Antibodies	>1 publications with CHST7 and Inflammation
Neoplasms, Experimental Antibodies	>1 publications with CHST7 and Neoplasms, Experimental
Necrosis Antibodies	>1 publications with CHST7 and Necrosis
Learning Disorders Antibodies	>1 publications with CHST7 and Learning Disorders

Organs/Tissues associated with CHST7 elisa kit

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>2 publications with CHST7 and Eye
Bone Marrow Antibodies	>1 publications with CHST7 and Bone Marrow
Mammary Gland Antibodies	>1 publications with CHST7 and Mammary Gland