Glutamic Acid Decarboxylase 65 (GAD65) ELISA Kit for Human

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GAD65 in Human serum, plasma. An antibody specific for GAD65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAD65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAD65 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAD65 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Intended Uses: For the quantitative detection of Human Glutamic acid decarboxylase 65 (GAD65) concentration in serum, plasma and other biological fluids.

Small volumes of GAD65 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS281731 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glutamic acid decarboxylase 65 (GAD65) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GAD65. The ELISA analytical biochemical technique of the MBS281731 kit is based on GAD65 antibody-GAD65 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GAD65 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GAD65. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).