Phosphoglycerate Mutase 1 (PGAM1) ELISA Kit for Human

Name: Human PGAM1 ELISA Kit; Phosphoglycerate Mutase 1; MyBioSource
Brand: MyBioSource
SKU: MBS281749
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281749

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PGAM1 in samples. An antibody specific for PGAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPGAM1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Phosphoglycerate mutase 1 (PGAM1), ELISA Kit

Full Product Name

Human Phosphoglycerate mutase 1 (PGAM1) ELISA Kit

Product Gene Name

PGAM1 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PGAM1. No significant cross-reactivity or interference between Human PGAM1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

28,804 Da

OMIM

J04173 mRNA

3D Structure

ModBase 3D Structure for P18669

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PGAM1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGAM1 purchase

MBS281749 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphoglycerate mutase 1 (PGAM1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PGAM1. The ELISA analytical biochemical technique of the MBS281749 kit is based on PGAM1 antibody-PGAM1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PGAM1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PGAM1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PGAM1. It may not necessarily be applicable to this product.

NCBI GI #

4505753

NCBI GeneID

5223

NCBI Accession #

NP_002620.1

UniProt Primary Accession #

P18669

UniProt Secondary Accession #

Q9BWC0

UniProt Related Accession #

P18669

GenBank Nucleotide #

NM_002629.3

NCBI Official Full Name

phosphoglycerate mutase 1 isoform 1

NCBI Official Synonym Full Names

phosphoglycerate mutase 1

NCBI Official Symbol

PGAM1

NCBI Official Synonym Symbols

PGAMA; PGAM-B; HEL-S-35

UniProt Protein Name

Phosphoglycerate mutase 1

UniProt Synonym Protein Names

BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B

Protein Family

Phosphoglycerate mutase

UniProt Gene Name

PGAM1

UniProt Synonym Gene Names

PGAMA; PGAM-B

NCBI Protein Information

phosphoglycerate mutase 1

NCBI Summary for PGAM1

The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

UniProt Comments for PGAM1

PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 3.1.3.13; EC 5.4.2.11; EC 5.4.2.4; Isomerase; Phosphatase (non-protein)

Chromosomal Location of Human Ortholog: 10q24.1

Cellular Component: cytoplasm; cytosol; extracellular exosome; extracellular region; ficolin-1-rich granule lumen; membrane; secretory granule lumen

Molecular Function: 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; hydrolase activity; phosphoglycerate mutase activity; protein binding; protein kinase binding

Biological Process: gluconeogenesis; glycolytic process; neutrophil degranulation; regulation of glycolysis; regulation of pentose-phosphate shunt; respiratory burst

Research Articles on PGAM1

1. When BPGM is knocked out, 1,3-BPG can directly phosphorylate PGAM1. In this case, PGAM1 phosphorylation and activity are decreased, but nevertheless sufficient to maintain normal glycolytic flux and cellular growth rate.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PGAM1 elisa kit

Reference Product	PubMed Publications
ENO1 elisa kit	>7 publications with PGAM1 and ENO1
PKM2 elisa kit	>4 publications with PGAM1 and PKM2
PGK1 elisa kit	>4 publications with PGAM1 and PGK1
TPI1 elisa kit	>3 publications with PGAM1 and TPI1
LDHA elisa kit	>2 publications with PGAM1 and LDHA

Pathways associated with PGAM1 elisa kit

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Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
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Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
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Central Carbon Metabolism In Cancer Pathway antibodies	Central Carbon Metabolism In Cancer Pathway Diagram
Central Carbon Metabolism In Cancer Pathway antibodies	Central Carbon Metabolism In Cancer Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Gluconeogenesis Pathway antibodies	Gluconeogenesis Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram

Diseases associated with PGAM1 elisa kit

Disease Name	Pubmed Publications
Carcinoma Antibodies	>4 publications with PGAM1 and Carcinoma
Disease Models, Animal Antibodies	>3 publications with PGAM1 and Disease Models, Animal
Carcinoma, Hepatocellular Antibodies	>1 publications with PGAM1 and Carcinoma, Hepatocellular
Hepatitis Antibodies	>1 publications with PGAM1 and Hepatitis
Hyperplasia Antibodies	>1 publications with PGAM1 and Hyperplasia
Liver Neoplasms Antibodies	>1 publications with PGAM1 and Liver Neoplasms
Necrosis Antibodies	>1 publications with PGAM1 and Necrosis
Carcinoma, Non-Small-Cell Lung Antibodies	>1 publications with PGAM1 and Carcinoma, Non-Small-Cell Lung
Mammary Neoplasms, Animal Antibodies	>1 publications with PGAM1 and Mammary Neoplasms, Animal
Carcinoma, Squamous Cell Antibodies	>1 publications with PGAM1 and Carcinoma, Squamous Cell

Organs/Tissues associated with PGAM1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>13 publications with PGAM1 and Brain
Blood Antibodies	>6 publications with PGAM1 and Blood
Muscle Antibodies	>4 publications with PGAM1 and Muscle
Testis Antibodies	>3 publications with PGAM1 and Testis
Lung Antibodies	>3 publications with PGAM1 and Lung
Heart Antibodies	>3 publications with PGAM1 and Heart
Liver Antibodies	>3 publications with PGAM1 and Liver
Ovary Antibodies	>2 publications with PGAM1 and Ovary
Kidney Antibodies	>2 publications with PGAM1 and Kidney
Stomach Antibodies	>1 publications with PGAM1 and Stomach