Probable G-Protein Coupled Receptor 133 (GPR133) ELISA Kit for Human

Name: Human GPR133 ELISA Kit; Probable G-Protein Coupled Receptor 133; MyBioSource
Brand: MyBioSource
SKU: MBS281934
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS281934

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPR133 in samples. An antibody specific for GPR133 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPR133 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Probable G-protein coupled receptor 133 (GPR133), ELISA Kit

Full Product Name

Human Probable G-protein coupled receptor 133 (GPR133) ELISA Kit

Product Gene Name

GPR133 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human GPR133. No significant cross-reactivity or interference between Human GPR133 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

99,984 Da

OMIM

AY532280 mRNA

3D Structure

ModBase 3D Structure for Q6QNK2

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GPR133 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPR133 purchase

MBS281934 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable G-protein coupled receptor 133 (GPR133) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPR133. The ELISA analytical biochemical technique of the MBS281934 kit is based on GPR133 antibody-GPR133 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPR133 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPR133. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GPR133. It may not necessarily be applicable to this product.

NCBI GI #

1059842860

NCBI GeneID

283383

NCBI Accession #

NP_001317426.1

UniProt Primary Accession #

Q6QNK2

UniProt Secondary Accession #

Q2M1L3; Q6ZMQ1; Q7Z7M2; Q86SM4; B2CKK9; B7ZLF7

UniProt Related Accession #

Q6QNK2

GenBank Nucleotide #

NM_001330497.1

NCBI Official Full Name

adhesion G-protein coupled receptor D1 isoform 2

NCBI Official Synonym Full Names

adhesion G protein-coupled receptor D1

NCBI Official Symbol

ADGRD1

NCBI Official Synonym Symbols

PGR25; GPR133

UniProt Protein Name

Adhesion G-protein coupled receptor D1

UniProt Synonym Protein Names

G-protein coupled receptor 133; G-protein coupled receptor PGR25

UniProt Gene Name

ADGRD1

UniProt Synonym Gene Names

GPR133; PGR25

NCBI Protein Information

adhesion G-protein coupled receptor D1

NCBI Summary for GPR133

The adhesion G-protein-coupled receptors (GPCRs), including GPR133, are membrane-bound proteins with long N termini containing multiple domains. GPCRs, or GPRs, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins (summary by Bjarnadottir et al., 2004 [PubMed 15203201]).[supplied by OMIM, Nov 2010]

UniProt Comments for GPR133

GPR133: Orphan receptor. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 2; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 12q24.33

Cellular Component: integral component of membrane; intracellular; plasma membrane

Molecular Function: G-protein coupled receptor activity

Biological Process: cell surface receptor signaling pathway; G-protein coupled receptor signaling pathway

Research Articles on GPR133

1. Studied GPR133's role in glioblastoma (GBM) in hypoxic conditions . Immunostaining revealed GPR133 expression is restricted to hypoxic regions of GBM and not present in normal brain. GPR133 knockdown depleted CD133+ GSCs and inhibited tumor sphere formation. GPR133 knockdown also prevented in vivo tumor formation and increased survival of implanted mice.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GPR133 elisa kit

Reference Product	PubMed Publications
GPR133 elisa kit	>3 publications with GPR133 and GPR133

Pathways associated with GPR133 elisa kit

Products by Pathway	Pathway Diagram
GPCRs, Other Pathway antibodies	GPCRs, Other Pathway Diagram

Diseases associated with GPR133 elisa kit

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>1 publications with GPR133 and Heart Diseases

Organs/Tissues associated with GPR133 elisa kit

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>1 publications with GPR133 and Heart
Brain Antibodies	>1 publications with GPR133 and Brain