Glyoxylate Reductase/Hydroxypyruvate Reductase (GRHPR) ELISA Kit for Human

Name: Human GRHPR ELISA Kit; Glyoxylate Reductase/Hydroxypyruvate Reductase; MyBioSource
Brand: MyBioSource
SKU: MBS282029
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282029

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GRHPR in samples. An antibody specific for GRHPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGRHPR present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Glyoxylate reductase/hydroxypyruvate reductase (GRHPR), ELISA Kit

Full Product Name

Human Glyoxylate reductase/hydroxypyruvate reductase (GRHPR) ELISA Kit

Product Gene Name

GRHPR elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human GRHPR. No significant cross-reactivity or interference between Human GRHPR and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

27,843 Da

OMIM

AF134895 mRNA

3D Structure

ModBase 3D Structure for Q9UBQ7

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GRHPR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GRHPR purchase

MBS282029 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glyoxylate reductase/hydroxypyruvate reductase (GRHPR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GRHPR. The ELISA analytical biochemical technique of the MBS282029 kit is based on GRHPR antibody-GRHPR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GRHPR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GRHPR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GRHPR. It may not necessarily be applicable to this product.

NCBI GI #

6912396

NCBI GeneID

9380

NCBI Accession #

NP_036335.1

UniProt Primary Accession #

Q9UBQ7

UniProt Secondary Accession #

Q5T945; Q9H3E9; Q9H636; Q9UKX1

UniProt Related Accession #

Q9UBQ7

GenBank Nucleotide #

NM_012203.1

NCBI Official Full Name

glyoxylate reductase/hydroxypyruvate reductase

NCBI Official Synonym Full Names

glyoxylate and hydroxypyruvate reductase

NCBI Official Symbol

GRHPR

NCBI Official Synonym Symbols

PH2; GLXR; GLYD

UniProt Protein Name

Glyoxylate reductase/hydroxypyruvate reductase

Protein Family

Glyoxylate reductase/hydroxypyruvate reductase

UniProt Gene Name

GRHPR

UniProt Synonym Gene Names

GLXR

NCBI Protein Information

glyoxylate reductase/hydroxypyruvate reductase

NCBI Summary for GRHPR

This gene encodes an enzyme with hydroxypyruvate reductase, glyoxylate reductase, and D-glycerate dehydrogenase enzymatic activities. The enzyme has widespread tissue expression and has a role in metabolism. Type II hyperoxaluria is caused by mutations in this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for GRHPR

GRHPR: Enzyme with hydroxy-pyruvate reductase, glyoxylate reductase and D-glycerate dehydrogenase enzymatic activities. Reduces hydroxypyruvate to D-glycerate, glyoxylate to glycolate oxidizes D-glycerate to hydroxypyruvate. Defects in GRHPR are the cause of hyperoxaluria primary type 2 (HP2); also known as primary hyperoxaluria type II (PH2). HP2 is a disorder where the main clinical manifestation is calcium oxalate nephrolithiasis though chronic as well as terminal renal insufficiency has been described. It is characterized by an elevated urinary excretion of oxalate and L- glycerate. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Protein type: Carbohydrate Metabolism - glyoxylate and dicarboxylate; Carbohydrate Metabolism - pyruvate; EC 1.1.1.79; EC 1.1.1.81; Oxidoreductase

Chromosomal Location of Human Ortholog: 9p13.2

Cellular Component: cytoplasm; cytosol; peroxisomal matrix

Molecular Function: carboxylic acid binding; glycerate dehydrogenase activity; glyoxylate reductase (NADP) activity; hydroxypyruvate reductase activity; NAD binding; protein homodimerization activity

Biological Process: dicarboxylic acid metabolic process; excretion; metabolic process; protein oligomerization

Disease: Hyperoxaluria, Primary, Type Ii

Research Articles on GRHPR

1. GRHPR was genotyped in Japanese patients with PH2 and all GRHPR mutations described to date were reviewed in terms of geographic and ethnic association

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GRHPR elisa kit

Reference Product	PubMed Publications
AGXT elisa kit	>11 publications with GRHPR and AGXT

Pathways associated with GRHPR elisa kit

Products by Pathway	Pathway Diagram
Glycine, Serine And Threonine Metabolism Pathway antibodies	Glycine, Serine And Threonine Metabolism Pathway Diagram
Glycine, Serine And Threonine Metabolism Pathway antibodies	Glycine, Serine And Threonine Metabolism Pathway Diagram
Glyoxylate And Dicarboxylate Metabolism Pathway antibodies	Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
Glyoxylate And Dicarboxylate Metabolism Pathway antibodies	Glyoxylate And Dicarboxylate Metabolism Pathway Diagram
Glyoxylate Metabolism Pathway antibodies	Glyoxylate Metabolism Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Pyruvate Metabolism Pathway antibodies	Pyruvate Metabolism Pathway Diagram
Pyruvate Metabolism Pathway antibodies	Pyruvate Metabolism Pathway Diagram

Diseases associated with GRHPR elisa kit

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>22 publications with GRHPR and Kidney Diseases
Primary hyperoxaluria type 2 Antibodies	>15 publications with GRHPR and Primary hyperoxaluria type 2
Liver Diseases Antibodies	>5 publications with GRHPR and Liver Diseases
Hypertension Antibodies	>4 publications with GRHPR and Hypertension
Cell Transformation, Neoplastic Antibodies	>1 publications with GRHPR and Cell Transformation, Neoplastic
Carcinoma, Hepatocellular Antibodies	>1 publications with GRHPR and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>1 publications with GRHPR and Liver Neoplasms
Inflammation Antibodies	>1 publications with GRHPR and Inflammation
Necrosis Antibodies	>1 publications with GRHPR and Necrosis

Organs/Tissues associated with GRHPR elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>16 publications with GRHPR and Kidney
Liver Antibodies	>15 publications with GRHPR and Liver
Blood Antibodies	>6 publications with GRHPR and Blood
Thyroid Antibodies	>1 publications with GRHPR and Thyroid
Bone Antibodies	>1 publications with GRHPR and Bone
Eye Antibodies	>1 publications with GRHPR and Eye
Skin Antibodies	>1 publications with GRHPR and Skin