Pre-Mrna-Splicing Factor RBM22 (RBM22) ELISA Kit for Human

Name: Human RBM22 ELISA Kit; Pre-Mrna-Splicing Factor RBM22; MyBioSource
Brand: MyBioSource
SKU: MBS282051
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282051

Intended Uses: For the quantitative detection of Human Pre-mRNA-splicing factor RBM22 (RBM22) concentration in serum, plasma, tissue homogenates and other biological fluids.Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Pre-mRNA-splicing factor RBM22, ELISA Kit

Full Product Name

Human Pre-mRNA-splicing factor RBM22 (RBM22) ELISA Kit

Product Gene Name

RBM22 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

62.5 pg/mL - 4000 pg/mL

Sensitivity

20.8 pg/mL (mean of 6 independent assays)

Specificity

This assay has high sensitivity and excellent specificity for detection of Human RBM22. No significant cross-reactivity or interference between Human RBM22 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

41,152 Da

OMIM

AL136933 mRNA

3D Structure

ModBase 3D Structure for Q9NW64

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of RBM22 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RBM22 purchase

MBS282051 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pre-mRNA-splicing factor RBM22, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RBM22. The ELISA analytical biochemical technique of the MBS282051 kit is based on RBM22 antibody-RBM22 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RBM22 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RBM22. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for RBM22. It may not necessarily be applicable to this product.

NCBI GI #

8922328

NCBI GeneID

55696

NCBI Accession #

NP_060517.1

UniProt Primary Accession #

Q9NW64

UniProt Secondary Accession #

O95607; A6NDM5; B4DLI9

UniProt Related Accession #

Q9NW64

GenBank Nucleotide #

NM_018047.2

NCBI Official Full Name

pre-mRNA-splicing factor RBM22

NCBI Official Synonym Full Names

RNA binding motif protein 22

NCBI Official Symbol

RBM22

NCBI Official Synonym Symbols

Cwc2; ZC3H16; fSAP47

UniProt Protein Name

Pre-mRNA-splicing factor RBM22

UniProt Synonym Protein Names

RNA-binding motif protein 22; Zinc finger CCCH domain-containing protein 16

Protein Family

Pre-mRNA-splicing factor

UniProt Gene Name

RBM22

UniProt Synonym Gene Names

ZC3H16

NCBI Protein Information

pre-mRNA-splicing factor RBM22

NCBI Summary for RBM22

This gene encodes an RNA binding protein. The encoded protein may play a role in cell division and may be involved in pre-mRNA splicing. Related pseudogenes exist on chromosomes 6, 7, 9, 13, 16, 18, and X. [provided by RefSeq, Mar 2009]

UniProt Comments for RBM22

RBM22: Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses. Belongs to the SLT11 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA processing; RNA splicing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: catalytic step 2 spliceosome; cytoplasm; nucleoplasm; nucleus; U2-type catalytic step 1 spliceosome; U2-type catalytic step 2 spliceosome

Molecular Function: calcium-dependent protein binding; metal ion binding; nucleocytoplasmic transporter activity; protein binding; RNA binding; U6 snRNA binding

Biological Process: mRNA cis splicing, via spliceosome; mRNA splicing, via spliceosome; positive regulation of intracellular protein transport; positive regulation of RNA splicing; protein import into nucleus, translocation; spliceosomal snRNP biogenesis

Research Articles on RBM22

1. Cwc2/RBM22-RNA contacts are functionally important.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with RBM22 elisa kit

Reference Product	PubMed Publications
SLU7 elisa kit	>1 publications with RBM22 and SLU7

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