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Wiskott-Aldrich Syndrome (WAS) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282069

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WAS in samples. An antibody specific for WAS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWAS present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Wiskott-Aldrich syndrome protein (WAS), ELISA Kit
Full Product Name
Mouse Wiskott-Aldrich syndrome protein (WAS) ELISA Kit
Product Gene Name
WAS elisa kit
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse WAS. No significant cross-reactivity or interference between Mouse WAS and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
54,192 Da
3D Structure
ModBase 3D Structure for P70315
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of WAS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WAS purchase

MBS282069 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Wiskott-Aldrich syndrome protein (WAS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WAS. The ELISA analytical biochemical technique of the MBS282069 kit is based on WAS antibody-WAS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WAS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WAS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for WAS. It may not necessarily be applicable to this product.

NCBI GI #
6678581
NCBI GeneID
22376
NCBI Accession #
NP_033541.1
UniProt Primary Accession #
P70315
UniProt Related Accession #
P70315
GenBank Nucleotide #
NM_009515.2
NCBI Official Full Name
wiskott-Aldrich syndrome protein homolog
NCBI Official Synonym Full Names
Wiskott-Aldrich syndrome
NCBI Official Symbol
Was
NCBI Official Synonym Symbols
Wasp; U42471
UniProt Protein Name
Wiskott-Aldrich syndrome protein homolog
Protein Family
Wiskott-Aldrich syndrome protein
UniProt Gene Name
Was
UniProt Synonym Gene Names
Wasp; WASp
NCBI Protein Information
wiskott-Aldrich syndrome protein homolog

UniProt Comments for WAS

WASP: a member of the Wiskott-Aldrich syndrome (WAS) family of proteins. A cytoplasmic protein expressed exclusively in hematopoietic cells. Transduces signals from surface receptors to the actin cytoskeleton. Associates with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Mutated in Wiskott-Aldrich syndrome, a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: X A1.1|X 3.65 cM

Cellular Component: actin filament; cytoplasm; cytoskeleton; cytosol; extracellular exosome; intercellular junction; phagocytic vesicle; vesicle membrane

Molecular Function: actin binding; identical protein binding; phospholipase binding; protein binding; protein kinase binding; Rac GTPase binding; SH3 domain binding; small GTPase binding

Biological Process: actin filament organization; actin filament polymerization; actin filament-based movement; actin polymerization or depolymerization; Cdc42 protein signal transduction; cellular response to interferon-gamma; endosomal transport; immune response; negative regulation of cell motility; negative regulation of stress fiber formation; positive regulation of Arp2/3 complex-mediated actin nucleation; regulation of actin polymerization and/or depolymerization; regulation of lamellipodium assembly; regulation of stress fiber assembly; regulation of T cell antigen processing and presentation; T cell activation
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Research Articles on WAS

1. DOCK8 and WASp are in the same signaling pathway that links T-cell receptors (TCRs) to the actin cytoskeleton in TCR-driven actin assembly.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with WAS elisa kit

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 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
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