Carbonic Anhydrase 12 (CA12) ELISA Kit for Mouse

Name: Mouse CA12 ELISA Kit; Carbonic Anhydrase 12; MyBioSource
Brand: MyBioSource
SKU: MBS282129
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282129

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CA12 in samples. An antibody specific for CA12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCA12 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Carbonic anhydrase 12 (CA12), ELISA Kit

Full Product Name

Mouse Carbonic anhydrase 12 (CA12) ELISA Kit

Product Gene Name

CA12 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse CA12. No significant cross-reactivity or interference between Mouse CA12 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

39,695 Da

3D Structure

ModBase 3D Structure for Q8CI85

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CA12 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CA12 purchase

MBS282129 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Carbonic anhydrase 12 (CA12) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CA12. The ELISA analytical biochemical technique of the MBS282129 kit is based on CA12 antibody-CA12 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CA12 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CA12. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CA12. It may not necessarily be applicable to this product.

NCBI GI #

807066356

NCBI GeneID

76459

NCBI Accession #

NP_001293077.1

UniProt Primary Accession #

Q8CI85

UniProt Secondary Accession #

Q8BKG6

UniProt Related Accession #

Q8CI85

GenBank Nucleotide #

NM_001306148.1

NCBI Official Full Name

carbonic anhydrase 12 isoform 2

NCBI Official Synonym Full Names

carbonic anhydrase 12

NCBI Official Symbol

Car12

NCBI Official Synonym Symbols

Ca12; CA-XII; AI314958; 2310047E01Rik

UniProt Protein Name

Carbonic anhydrase 12

UniProt Synonym Protein Names

Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII

Protein Family

Carbonic anhydrase

UniProt Gene Name

Ca12

UniProt Synonym Gene Names

Car12; CA-XII

NCBI Protein Information

carbonic anhydrase 12

NCBI Summary for CA12

This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

UniProt Comments for CA12

CA12: Reversible hydration of carbon dioxide. Homodimer. Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA), benzenesulfonamide and derivatives (4-carboxyethylbenzene-sulfonamide, 4- carboxyethylbenzene-sulfonamide ethyl ester, 4-(acetyl-2- aminoethyl)benzene-sulfonamide, 4-aminoethylbenzene-sulfonamide) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 9|9 C

Cellular Component: integral component of membrane; membrane

Molecular Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding

Biological Process: carbon dioxide transport; chloride ion homeostasis; one-carbon metabolic process; regulation of pH

Research Articles on CA12

1. P2Y2R-mediated expression of CAXII by VICs acidifies the extracellular space and promotes the regression of CAVS.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CA12 elisa kit

Reference Product	PubMed Publications
Slc4a4 elisa kit	>1 publications with CA12 and Slc4a4

Pathways associated with CA12 elisa kit

Products by Pathway	Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Nitrogen Metabolism Pathway antibodies	Nitrogen Metabolism Pathway Diagram
Nitrogen Metabolism Pathway antibodies	Nitrogen Metabolism Pathway Diagram
Reversible Hydration Of Carbon Dioxide Pathway antibodies	Reversible Hydration Of Carbon Dioxide Pathway Diagram

Diseases associated with CA12 elisa kit

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>1 publications with CA12 and Kidney Diseases
Male Urogenital Diseases Antibodies	>1 publications with CA12 and Male Urogenital Diseases

Organs/Tissues associated with CA12 elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>2 publications with CA12 and Kidney
Eye Antibodies	>1 publications with CA12 and Eye
Brain Antibodies	>1 publications with CA12 and Brain
Intestine Antibodies	>1 publications with CA12 and Intestine
Inner Ear Antibodies	>1 publications with CA12 and Inner Ear