Probable ATP-Dependent RNA Helicase DHX40 (DHX40) ELISA Kit for Human

DHX40 is a member of the DEAH box family of ATP-dependent RNA helicases. RNA helicases catalyze the unwinding of double-stranded RNA and play a role in RNA metabolism, including pre-mRNA splicing, ribosome biogenesis, and organellar gene expression.Xu et al. (2002) cloned DHX40, which they called DDX40, during large-scale cDNA sequencing of clones obtained from a fetal brain cDNA library. The deduced 779-amino acid protein has a calculated molecular mass of 86 kD. DHX40 has a well-conserved DEAH box region and shares high amino acid identity with DHX8 and the yeast proteins Prp22 and Dhr1, both of which function in RNA processing. Northern blot analysis detected ubiquitous expression of a 3.6-kb DHX40 transcript.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DHX40 in samples. An antibody specific for DHX40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHX40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DHX40 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DHX40 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of DHX40 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS282148 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable ATP-dependent RNA helicase DHX40, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DHX40. The ELISA analytical biochemical technique of the MBS282148 kit is based on DHX40 antibody-DHX40 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHX40 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHX40. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).