Histone H3.3 (H3F3A) ELISA Kit for Pig

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate H3F3A in samples. An antibody specific for H3F3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyH3F3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for H3F3A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of H3F3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of H3F3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS282201 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Histone H3.3 (H3F3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing H3F3A. The ELISA analytical biochemical technique of the MBS282201 kit is based on H3F3A antibody-H3F3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect H3F3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, H3F3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).