Glycosaminoglycan Xylosylkinase (FAM20B) ELISA Kit for Human

Name: Human FAM20B ELISA Kit; Glycosaminoglycan Xylosylkinase; MyBioSource
Brand: MyBioSource
SKU: MBS282231
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282231

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate FAM20B in samples. An antibody specific for FAM20B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFAM20B present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Glycosaminoglycan xylosylkinase (FAM20B), ELISA Kit

Full Product Name

Human Glycosaminoglycan xylosylkinase (FAM20B) ELISA Kit

Product Gene Name

FAM20B elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human FAM20B. No significant cross-reactivity or interference between Human FAM20B and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

46,432 Da

OMIM

AB480690 mRNA

3D Structure

ModBase 3D Structure for O75063

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of FAM20B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FAM20B purchase

MBS282231 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glycosaminoglycan xylosylkinase (FAM20B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FAM20B. The ELISA analytical biochemical technique of the MBS282231 kit is based on FAM20B antibody-FAM20B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FAM20B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FAM20B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FAM20B. It may not necessarily be applicable to this product.

NCBI GI #

1024249351

NCBI GeneID

9917

NCBI Accession #

NP_001311239.1

UniProt Primary Accession #

O75063

UniProt Secondary Accession #

Q5W0C3; Q5W0C4

UniProt Related Accession #

O75063

GenBank Nucleotide #

NM_001324310.1

NCBI Official Full Name

glycosaminoglycan xylosylkinase

NCBI Official Synonym Full Names

FAM20B, glycosaminoglycan xylosylkinase

NCBI Official Symbol

FAM20B

NCBI Official Synonym Symbols

gxk1

UniProt Protein Name

Glycosaminoglycan xylosylkinase

UniProt Synonym Protein Names

Xylose kinase

Protein Family

Glycosaminoglycan xylosylkinase

UniProt Gene Name

FAM20B

NCBI Protein Information

glycosaminoglycan xylosylkinase

UniProt Comments for FAM20B

FAM20B: Responsible for the 2-O-phosphorylation of xylose in the glycosaminoglycan-protein linkage region of proteoglycans thereby regulating the amount of mature GAG chains. Sulfated glycosaminoglycans (GAGs), including heparan sulfate and chondroitin sulfate, are synthesized on the so-called common GAG- protein linkage region (GlcUAbeta1-3Galbeta1-3Galbeta1-4Xylbeta1- O-Ser) of core proteins, which is formed by the stepwise addition of monosaccharide residues by the respective specific glycosyltransferases. Xylose 2-o-phosphorylation may influence the catalytic activity of B3GAT3 (GlcAT-I) which completes the precursor tetrasaccharide of GAG-protein linkage regions on which the repeating disaccharide region is synthesized. Belongs to the FAM20 family.

Protein type: EC 2.7.1.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: extracellular space; Golgi apparatus; Golgi membrane; integral component of membrane; nucleoplasm

Molecular Function: ATP binding; kinase activity; metal ion binding; phosphotransferase activity, alcohol group as acceptor; protein binding

Biological Process: phosphorylation; positive regulation of protein targeting to mitochondrion

Research Articles on FAM20B

1. Observational study of gene-disease association. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with FAM20B elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>5 publications with FAM20B and Bone
Liver Antibodies	>2 publications with FAM20B and Liver
Kidney Antibodies	>1 publications with FAM20B and Kidney
Mouth Antibodies	>1 publications with FAM20B and Mouth
Connective Tissue Antibodies	>1 publications with FAM20B and Connective Tissue
Muscle Antibodies	>1 publications with FAM20B and Muscle
Skin Antibodies	>1 publications with FAM20B and Skin