Protein-Tyrosine Kinase 2-Beta (PTK2B) ELISA Kit for Human

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Pyk2: a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described.

Protein type: EC 2.7.10.2; Fak family; Kinase, protein; Nuclear receptor co-regulator; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: axon; cell body; cell cortex; cell soma; cytoplasm; cytoskeleton; cytosol; dendrite; dendritic spine; extrinsic component of cytoplasmic side of plasma membrane; focal adhesion; growth cone; lamellipodium; membrane raft; NMDA selective glutamate receptor complex; nucleus; perinuclear region of cytoplasm; postsynaptic density

Molecular Function: 3-phosphoinositide-dependent protein kinase binding; ATP binding; calmodulin-dependent protein kinase activity; NMDA glutamate receptor activity; non-membrane spanning protein tyrosine kinase activity; protein binding; protein complex binding; protein tyrosine kinase activity; receptor binding; signal transducer activity; ubiquitin protein ligase binding

Biological Process: activation of JAK protein; adaptive immune response; angiogenesis; apoptosis; blood vessel endothelial cell migration; bone resorption; cell surface receptor signaling pathway; cellular defense response; cellular response to fluid shear stress; cellular response to retinoic acid; chemokine-mediated signaling pathway; elevation of cytosolic calcium ion concentration; epidermal growth factor receptor signaling pathway; focal adhesion assembly; glial cell proliferation; innate immune response; integrin-mediated signaling pathway; interleukin-7-mediated signaling pathway; ionotropic glutamate receptor signaling pathway; MAPK cascade; marginal zone B cell differentiation; negative regulation of apoptosis; negative regulation of bone mineralization; negative regulation of cell proliferation; negative regulation of muscle cell apoptotic process; negative regulation of myeloid cell differentiation; negative regulation of neuron apoptosis; negative regulation of potassium ion transport; neuron projection development; oocyte maturation; peptidyl-tyrosine autophosphorylation; peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of angiogenesis; positive regulation of B cell chemotaxis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of DNA biosynthetic process; positive regulation of endothelial cell migration; positive regulation of ERK1 and ERK2 cascade; positive regulation of excitatory postsynaptic potential; positive regulation of JNK cascade; positive regulation of JUN kinase activity; positive regulation of neuron projection development; positive regulation of nitric oxide biosynthetic process; positive regulation of nitric-oxide synthase activity; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphatidylinositol 3-kinase activity; positive regulation of protein kinase activity; positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process; positive regulation of synaptic transmission, glutamatergic; positive regulation of translation; protein amino acid phosphorylation; protein autophosphorylation; protein complex assembly; regulation of actin cytoskeleton reorganization; regulation of calcium-mediated signaling; regulation of cell adhesion; regulation of cell shape; regulation of cGMP biosynthetic process; regulation of cGMP-mediated signaling; regulation of establishment of cell polarity; regulation of inositol trisphosphate biosynthetic process; regulation of macrophage chemotaxis; regulation of NMDA receptor activity; regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process; regulation of release of sequestered calcium ion into cytosol; regulation of synaptic plasticity; response to calcium ion; response to cAMP; response to cocaine; response to drug; response to ethanol; response to glucose stimulus; response to hormone; response to hydrogen peroxide; response to hypoxia; response to lithium ion; response to mechanical stimulus; response to osmotic stress; response to stress; signal complex assembly; signal transduction; sprouting angiogenesis; stress fiber assembly; tumor necrosis factor-mediated signaling pathway; vascular endothelial growth factor receptor signaling pathway