Cardiotrophin-Like Cytokine Factor 1 (CLCF1) ELISA Kit for Mouse

Name: Mouse CLCF1 ELISA Kit; Cardiotrophin-Like Cytokine Factor 1; MyBioSource
Brand: MyBioSource
SKU: MBS282234
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282234

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CLCF1 in samples. An antibody specific for CLCF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCLCF1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Cardiotrophin-like cytokine factor 1 (CLCF1), ELISA Kit

Full Product Name

Mouse Cardiotrophin-like cytokine factor 1 (CLCF1) ELISA Kit

Product Gene Name

CLCF1 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse CLCF1. No significant cross-reactivity or interference between Mouse CLCF1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

25,261 Da

3D Structure

ModBase 3D Structure for Q9QZM3

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of CLCF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CLCF1 purchase

MBS282234 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cardiotrophin-like cytokine factor 1 (CLCF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CLCF1. The ELISA analytical biochemical technique of the MBS282234 kit is based on CLCF1 antibody-CLCF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CLCF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CLCF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CLCF1. It may not necessarily be applicable to this product.

NCBI GI #

845634607

NCBI GeneID

56708

NCBI Accession #

NP_001296967.1

UniProt Primary Accession #

Q9QZM3

UniProt Secondary Accession #

Q3SXK2

UniProt Related Accession #

Q9QZM3

GenBank Nucleotide #

NM_001310038.2

NCBI Official Full Name

cardiotrophin-like cytokine factor 1 isoform 2

NCBI Official Synonym Full Names

cardiotrophin-like cytokine factor 1

NCBI Official Symbol

Clcf1

NCBI Official Synonym Symbols

CLC; Bsf3; BSF-3; NNT-1

UniProt Protein Name

Cardiotrophin-like cytokine factor 1

UniProt Synonym Protein Names

B-cell-stimulating factor 3; BSF-3; Novel neurotrophin-1; NNT-1

Protein Family

Cardiotrophin-like cytokine factor

UniProt Gene Name

Clcf1

UniProt Synonym Gene Names

Bsf3; Nnt1; BSF-3; NNT-1

NCBI Protein Information

cardiotrophin-like cytokine factor 1

UniProt Comments for CLCF1

CLCF1: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19|19 A

Cellular Component: extracellular region; extracellular space; intracellular

Molecular Function: ciliary neurotrophic factor receptor binding; cytokine activity; protein binding; protein heterodimerization activity; receptor binding

Biological Process: B cell differentiation; cell surface receptor signaling pathway; JAK-STAT cascade; negative regulation of neuron apoptosis; positive regulation of astrocyte differentiation; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of corticotropin-releasing hormone secretion; positive regulation of immunoglobulin production; positive regulation of isotype switching to IgE isotypes; positive regulation of type 2 immune response; positive regulation of tyrosine phosphorylation of STAT protein; regulation of inflammatory response; regulation of protein kinase B signaling

Research Articles on CLCF1

1. Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CLCF1 elisa kit

Reference Product	PubMed Publications
Crlf1 elisa kit	>12 publications with CLCF1 and Crlf1
Cntfr elisa kit	>3 publications with CLCF1 and Cntfr
Park7 elisa kit	>1 publications with CLCF1 and Park7

Pathways associated with CLCF1 elisa kit

Products by Pathway	Pathway Diagram
Cytokine Signaling In Immune System Pathway antibodies	Cytokine Signaling In Immune System Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
IL-6-type Cytokine Receptor Ligand Interactions Pathway antibodies	IL-6-type Cytokine Receptor Ligand Interactions Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Interleukin-6 Family Signaling Pathway antibodies	Interleukin-6 Family Signaling Pathway Diagram
Signaling By Interleukins Pathway antibodies	Signaling By Interleukins Pathway Diagram

Diseases associated with CLCF1 elisa kit

Disease Name	Pubmed Publications
Cold-Induced Sweating Syndrome 1 Antibodies	>5 publications with CLCF1 and Cold-Induced Sweating Syndrome 1
Seizures Antibodies	>2 publications with CLCF1 and Seizures
Cardiovascular Diseases Antibodies	>2 publications with CLCF1 and Cardiovascular Diseases
Inflammation Antibodies	>2 publications with CLCF1 and Inflammation
Nerve Degeneration Antibodies	>1 publications with CLCF1 and Nerve Degeneration
Lung Neoplasms Antibodies	>1 publications with CLCF1 and Lung Neoplasms
Lung Diseases Antibodies	>1 publications with CLCF1 and Lung Diseases
Fibrosis Antibodies	>1 publications with CLCF1 and Fibrosis
Kidney Diseases Antibodies	>1 publications with CLCF1 and Kidney Diseases
Necrosis Antibodies	>1 publications with CLCF1 and Necrosis

Organs/Tissues associated with CLCF1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with CLCF1 and Brain
Liver Antibodies	>1 publications with CLCF1 and Liver
Lung Antibodies	>1 publications with CLCF1 and Lung