Hamster PL-A2 ELISA Kit – Cat# MBS028224

Phospholipase A2 (PL-A2) ELISA Kit for Hamster

Name: Hamster PL-A2 ELISA Kit; Phospholipase A2; MyBioSource
Brand: MyBioSource
SKU: MBS028224
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS028224

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of PLA2 (hereafter termed "analyte") in undiluted original… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Phospholipase A2 (PL-A2), ELISA Kit

Full Product Name

Hamster Phospholipase A2 ELISA Kit

Product Gene Name

PL-A2 elisa kit

Species Reactivity

Hamster

Assay Type

Quantitative Sandwich

Detection Range

3.12ng/ml-100ng/ml

Sensitivity

1.0ng/ml

Specificity

No significant cross-reactivity or interference between this analyte and analogues is observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

16,294 Da

Chromosome Location

Chromosome: 5; NC_005104.3 (161024073..161026650). Location: 5q36

3D Structure

ModBase 3D Structure for P14423

Other Notes

Small volumes of PL-A2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PL-A2 purchase

MBS028224 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phospholipase A2 (PL-A2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PL-A2. The ELISA analytical biochemical technique of the MBS028224 kit is based on PL-A2 antibody-PL-A2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PL-A2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PL-A2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PL-A2. It may not necessarily be applicable to this product.

NCBI GI #

291621686

NCBI GeneID

29692

NCBI Accession #

NP_113786.3

UniProt Primary Accession #

P14423

UniProt Related Accession #

P14423

GenBank Nucleotide #

NM_031598.3

NCBI Official Full Name

phospholipase A2, membrane associated

NCBI Official Synonym Full Names

phospholipase A2, group IIA (platelets, synovial fluid)

NCBI Official Symbol

Pla2g2a

NCBI Official Synonym Symbols

sPLA2

UniProt Protein Name

Phospholipase A2, membrane associated

UniProt Synonym Protein Names

GIIC sPLA2; Group IIA phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A

Protein Family

Phospholipase A2 A2-actitoxin

UniProt Gene Name

Pla2g2a

UniProt Entry Name

PA2GA_RAT

NCBI Protein Information

phospholipase A2, membrane associated; GIIC sPLA2; platelet phospholipase A2; group IIA phospholipase A2; eted enzyme type IIA phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A

NCBI Summary for PL-A2

hydrolyzes acyl group at the sn-2 position of phospholipids, forming non-esterified fatty acids and lysophospholipids; plays a critical role in inflammation; induces proliferation of smooth muscle cells [RGD, Feb 2006]

UniProt Comments for PL-A2

Function: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Catalytic activity: Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Cofactor: Binds 1 calcium ion per subunit

By similarity.

Subcellular location: Membrane; Peripheral membrane protein.

Miscellaneous: Group II phospholipase A2 is found in many cells and also extracellularly. The membrane-bound and secreted forms are identical and are encoded by a single gene.

Sequence similarities: Belongs to the phospholipase A2 family.

Research Articles on PL-A2

1. Secreted PLA2-IIA regulates the permeability of microvascular endothelial cells during brain inflammation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PL-A2 elisa kit

Reference Product	PubMed Publications
Pla2g4a elisa kit	>6 publications with PL-A2 and Pla2g4a
Il6 elisa kit	>3 publications with PL-A2 and Il6
Ptgs2 elisa kit	>3 publications with PL-A2 and Ptgs2
Ptgs1 elisa kit	>2 publications with PL-A2 and Ptgs1

Pathways associated with PL-A2 elisa kit

Products by Pathway	Pathway Diagram
Acyl Chain Remodelling Of PC Pathway antibodies	Acyl Chain Remodelling Of PC Pathway Diagram
Acyl Chain Remodelling Of PE Pathway antibodies	Acyl Chain Remodelling Of PE Pathway Diagram
Acyl Chain Remodelling Of PG Pathway antibodies	Acyl Chain Remodelling Of PG Pathway Diagram
Acyl Chain Remodelling Of PI Pathway antibodies	Acyl Chain Remodelling Of PI Pathway Diagram
Acyl Chain Remodelling Of PS Pathway antibodies	Acyl Chain Remodelling Of PS Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Arachidonic Acid Metabolism Pathway antibodies	Arachidonic Acid Metabolism Pathway Diagram
Eicosanoid Synthesis Pathway antibodies	Eicosanoid Synthesis Pathway Diagram
Ether Lipid Metabolism Pathway antibodies	Ether Lipid Metabolism Pathway Diagram
Ether Lipid Metabolism Pathway antibodies	Ether Lipid Metabolism Pathway Diagram

Diseases associated with PL-A2 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>55 publications with PL-A2 and Neoplasms
Inflammation Antibodies	>27 publications with PL-A2 and Inflammation
Colorectal Neoplasms Antibodies	>24 publications with PL-A2 and Colorectal Neoplasms
Carcinoma Antibodies	>15 publications with PL-A2 and Carcinoma
Heart Diseases Antibodies	>6 publications with PL-A2 and Heart Diseases
Atherosclerosis Antibodies	>6 publications with PL-A2 and Atherosclerosis
Necrosis Antibodies	>5 publications with PL-A2 and Necrosis
Lung Neoplasms Antibodies	>5 publications with PL-A2 and Lung Neoplasms
Myocardial Ischemia Antibodies	>4 publications with PL-A2 and Myocardial Ischemia
Colitis Antibodies	>4 publications with PL-A2 and Colitis

Organs/Tissues associated with PL-A2 elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>8 publications with PL-A2 and Lung
Heart Antibodies	>8 publications with PL-A2 and Heart
Liver Antibodies	>5 publications with PL-A2 and Liver
Prostate Antibodies	>4 publications with PL-A2 and Prostate
Artery Antibodies	>2 publications with PL-A2 and Artery
Pancreas Antibodies	>1 publications with PL-A2 and Pancreas
Embryonic Tissue Antibodies	>1 publications with PL-A2 and Embryonic Tissue