Wd Repeat-Containing Protein 61 (WDR61) ELISA Kit for Mouse

Name: Mouse WDR61 ELISA Kit; Wd Repeat-Containing Protein 61; MyBioSource
Brand: MyBioSource
SKU: MBS282243
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282243

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WDR61 in samples. An antibody specific for WDR61 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWDR61 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

WD repeat-containing protein 61 (WDR61), ELISA Kit

Full Product Name

Mouse WD repeat-containing protein 61 (WDR61) ELISA Kit

Product Gene Name

WDR61 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse WDR61. No significant cross-reactivity or interference between Mouse WDR61 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

33,773 Da

3D Structure

ModBase 3D Structure for Q9ERF3

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of WDR61 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WDR61 purchase

MBS282243 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the WD repeat-containing protein 61 (WDR61) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WDR61. The ELISA analytical biochemical technique of the MBS282243 kit is based on WDR61 antibody-WDR61 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WDR61 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WDR61. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for WDR61. It may not necessarily be applicable to this product.

NCBI GI #

70778817

NCBI GeneID

66317

NCBI Accession #

NP_001020546.1

UniProt Primary Accession #

Q9ERF3

UniProt Secondary Accession #

Q3U562; Q9CZP1

UniProt Related Accession #

Q9ERF3

GenBank Nucleotide #

NM_001025375.2

NCBI Official Full Name

WD repeat-containing protein 61 isoform a

NCBI Official Synonym Full Names

WD repeat domain 61

NCBI Official Symbol

Wdr61

NCBI Official Synonym Symbols

REC14; 2700038L12Rik; 2810418I05Rik

UniProt Protein Name

WD repeat-containing protein 61

UniProt Synonym Protein Names

Meiotic recombination REC14 protein homolog

Protein Family

WD repeat-containing protein

UniProt Gene Name

Wdr61

NCBI Protein Information

WD repeat-containing protein 61

UniProt Comments for WDR61

WDR61: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.

Chromosomal Location of Human Ortholog: 9|9 A5.3

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Biological Process: negative regulation of myeloid cell differentiation; positive regulation of histone H3-K4 methylation; positive regulation of transcription elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription, DNA-dependent; Wnt signaling pathway

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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