Wiskott-Aldrich Syndrome Protein Family Member 1 (WASF1) ELISA Kit for Mouse

Name: Mouse WASF1 ELISA Kit; Wiskott-Aldrich Syndrome Protein Family Member 1; MyBioSource
Brand: MyBioSource
SKU: MBS282278
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282278

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WASF1 in samples. An antibody specific for WASF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWASF1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Wiskott-Aldrich syndrome protein family member 1 (WASF1), ELISA Kit

Full Product Name

Mouse Wiskott-Aldrich syndrome protein family member 1 (WASF1) ELISA Kit

Product Gene Name

WASF1 elisa kit

Species Reactivity

Mouse

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse WASF1. No significant cross-reactivity or interference between Mouse WASF1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

61,509 Da

3D Structure

ModBase 3D Structure for Q8R5H6

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of WASF1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WASF1 purchase

MBS282278 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Wiskott-Aldrich syndrome protein family member 1 (WASF1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WASF1. The ELISA analytical biochemical technique of the MBS282278 kit is based on WASF1 antibody-WASF1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WASF1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WASF1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for WASF1. It may not necessarily be applicable to this product.

NCBI GI #

13994209

NCBI GeneID

83767

NCBI Accession #

NP_114083.1

UniProt Primary Accession #

Q8R5H6

UniProt Secondary Accession #

Q91W51; Q9ERQ9

UniProt Related Accession #

Q8R5H6

GenBank Nucleotide #

NM_031877.3

NCBI Official Full Name

wiskott-Aldrich syndrome protein family member 1

NCBI Official Synonym Full Names

WAS protein family, member 1

NCBI Official Symbol

Wasf1

NCBI Official Synonym Symbols

Scar; WAVE; WAVE-1; AI195380; AI838537

UniProt Protein Name

Wiskott-Aldrich syndrome protein family member 1

UniProt Synonym Protein Names

Protein WAVE-1

Protein Family

Wiskott-Aldrich syndrome protein family

UniProt Gene Name

Wasf1

UniProt Synonym Gene Names

Wave1; WASP family protein member 1

NCBI Protein Information

wiskott-Aldrich syndrome protein family member 1

UniProt Comments for WASF1

WAVE1: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Belongs to the SCAR/WAVE family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10 B1|10 22.07 cM

Cellular Component: cell junction; cytoplasm; cytoskeleton; lamellipodium; mitochondrial outer membrane; mitochondrion; protein complex; synapse

Molecular Function: actin binding; protein binding; protein complex binding; protein kinase A binding; Rac GTPase binding

Biological Process: actin cytoskeleton organization; cellular response to brain-derived neurotrophic factor stimulus; lamellipodium morphogenesis; neuron projection development; positive regulation of Arp2/3 complex-mediated actin nucleation; positive regulation of nerve growth factor receptor signaling pathway; Rac protein signal transduction; receptor-mediated endocytosis

Research Articles on WASF1

1. We identified a phosphorylation-dependent mechanism that regulates selective recruitment of these effectors to Lamellipodin: Abl-mediated Lamellipodin phosphorylation promotes its association with both Scar/WAVE and Ena/VASP, whereas Src-dependent phosphorylation enhances binding to Scar/WAVE but not to Ena/VASP

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with WASF1 elisa kit

Reference Product	PubMed Publications
Baiap2 elisa kit	>2 publications with WASF1 and Baiap2

Pathways associated with WASF1 elisa kit

Products by Pathway	Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Fc Gamma R-mediated Phagocytosis Pathway antibodies	Fc Gamma R-mediated Phagocytosis Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram
Regulation Of Actin Cytoskeleton Pathway antibodies	Regulation Of Actin Cytoskeleton Pathway Diagram
Salmonella Infection Pathway antibodies	Salmonella Infection Pathway Diagram

Diseases associated with WASF1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>14 publications with WASF1 and Neoplasms
Blood Coagulation Disorders Antibodies	>6 publications with WASF1 and Blood Coagulation Disorders
Precursor Cell Lymphoblastic Leukemia-Lymphoma Antibodies	>2 publications with WASF1 and Precursor Cell Lymphoblastic Leukemia-Lymphoma
Lung Neoplasms Antibodies	>2 publications with WASF1 and Lung Neoplasms
Melanoma Antibodies	>2 publications with WASF1 and Melanoma
Congenital Abnormalities Antibodies	>1 publications with WASF1 and Congenital Abnormalities
Melanoma, Experimental Antibodies	>1 publications with WASF1 and Melanoma, Experimental
Genital Diseases, Male Antibodies	>1 publications with WASF1 and Genital Diseases, Male
Prostatic Neoplasms Antibodies	>1 publications with WASF1 and Prostatic Neoplasms
Stomach Diseases Antibodies	>1 publications with WASF1 and Stomach Diseases

Organs/Tissues associated with WASF1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>14 publications with WASF1 and Brain
Lung Antibodies	>2 publications with WASF1 and Lung
Skin Antibodies	>1 publications with WASF1 and Skin
Kidney Antibodies	>1 publications with WASF1 and Kidney
Heart Antibodies	>1 publications with WASF1 and Heart
Pancreas Antibodies	>1 publications with WASF1 and Pancreas
Embryonic Tissue Antibodies	>1 publications with WASF1 and Embryonic Tissue