Human PIP5K1C ELISA Kit – Cat# MBS282297

Phosphatidylinositol-4-Phosphate 5-Kinase Type-1 Gamma (PIP5K1C) ELISA Kit for Human

Name: Human PIP5K1C ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS282297
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282297

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PIP5K1C in Human serum, plasma. An antibody specific for PIP5K1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIP5K1C… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (PIP5K1C), ELISA Kit

Full Product Name

Human Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (PIP5K1C) ELISA Kit

Product Gene Name

PIP5K1C elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma and other biological fluids

Detection Range

31.25 pg/mL - 2000 pg/mL

Sensitivity

15.6 pg/mL (mean of 6 independent assays)

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PIP5K1C. No significant cross-reactivity or interference between Human PIP5K1C and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

70,214 Da

OMIM

FJ965536 mRNA

3D Structure

ModBase 3D Structure for O60331

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PIP5K1C elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIP5K1C purchase

MBS282297 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (PIP5K1C) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PIP5K1C. The ELISA analytical biochemical technique of the MBS282297 kit is based on PIP5K1C antibody-PIP5K1C antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PIP5K1C antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PIP5K1C. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PIP5K1C. It may not necessarily be applicable to this product.

NCBI GI #

307691174

NCBI GeneID

23396

NCBI Accession #

NP_001182662.1

UniProt Primary Accession #

O60331

UniProt Secondary Accession #

Q7LE07; B7Z9E7; C6GIJ7; C6GIJ8

UniProt Related Accession #

O60331

GenBank Nucleotide #

NM_001195733.1

NCBI Official Full Name

phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform 1

NCBI Official Synonym Full Names

phosphatidylinositol-4-phosphate 5-kinase type 1 gamma

NCBI Official Symbol

PIP5K1C

NCBI Official Synonym Symbols

LCCS3; PIP5Kgamma; PIP5K-GAMMA; PIP5K1-gamma

UniProt Protein Name

Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma

UniProt Synonym Protein Names

Phosphatidylinositol 4-phosphate 5-kinase type I gamma; PIP5KIgamma

Protein Family

Phosphatidylinositol 4-phosphate 5-kinase

UniProt Gene Name

PIP5K1C

UniProt Synonym Gene Names

KIAA0589; PIP5K1-gamma; PtdIns(4)P-5-kinase 1 gamma; PIP5KIgamma

NCBI Protein Information

phosphatidylinositol 4-phosphate 5-kinase type-1 gamma

NCBI Summary for PIP5K1C

This locus encodes a type I phosphatidylinositol 4-phosphate 5-kinase. The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. This enzyme is found at synapses and has been found to play roles in endocytosis and cell migration. Mutations at this locus have been associated with lethal congenital contractural syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Sep 2010]

UniProt Comments for PIP5K1C

PIP5K1C: a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.

Protein type: Carbohydrate Metabolism - inositol phosphate; EC 2.7.1.68; Kinase, lipid; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytosol; endosome membrane; focal adhesion; nucleoplasm; phagocytic cup; presynapse; uropod

Molecular Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; ATP binding; protein binding

Biological Process: actin cytoskeleton organization; clathrin-dependent endocytosis; neutrophil chemotaxis; phagocytosis; phosphatidylinositol biosynthetic process; phosphatidylinositol phosphorylation; regulation of phosphatidylinositol 3-kinase signaling; synaptic vesicle endocytosis; synaptic vesicle exocytosis

Disease: Lethal Congenital Contracture Syndrome 3

Research Articles on PIP5K1C

1. FAK sustained the active integrin conformation by maintaining talin association with Rab11 endosomes in a type I phosphatidylinositol phosphate kinase (PIPKIgamma)-dependent manner.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PIP5K1C elisa kit

Products by Pathway	Pathway Diagram
3-phosphoinositide Biosynthesis Pathway antibodies	3-phosphoinositide Biosynthesis Pathway Diagram
3-phosphoinositide Biosynthesis Pathway antibodies	3-phosphoinositide Biosynthesis Pathway Diagram
Arf6 Trafficking Events Pathway antibodies	Arf6 Trafficking Events Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway antibodies	D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway Diagram
D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway antibodies	D-myo-inositol (1,4,5)-trisphosphate Biosynthesis Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
E-cadherin Signaling In The Nascent Adherens Junction Pathway antibodies	E-cadherin Signaling In The Nascent Adherens Junction Pathway Diagram
EGF Receptor (ErbB1) Signaling Pathway antibodies	EGF Receptor (ErbB1) Signaling Pathway Diagram

Diseases associated with PIP5K1C elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>1 publications with PIP5K1C and Inflammation
Lethal Congenital Contractural Syndrome 3 Antibodies	>1 publications with PIP5K1C and Lethal Congenital Contractural Syndrome 3

Organs/Tissues associated with PIP5K1C elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>2 publications with PIP5K1C and Blood
Bladder Antibodies	>1 publications with PIP5K1C and Bladder
Vascular Antibodies	>1 publications with PIP5K1C and Vascular
Testis Antibodies	>1 publications with PIP5K1C and Testis