GMP Synthase (GMPS) ELISA Kit for Human

Name: Human GMPS ELISA Kit; GMP Synthase; MyBioSource
Brand: MyBioSource
SKU: MBS282332
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282332

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GMPS in samples. An antibody specific for GMPS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGMPS present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

GMP synthase (GMPS), ELISA Kit

Full Product Name

Human GMP synthase (GMPS) ELISA Kit

Product Gene Name

GMPS elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human GMPS. No significant cross-reactivity or interference between Human GMPS and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

65,929 Da

OMIM

U10860 mRNA

3D Structure

ModBase 3D Structure for P49915

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GMPS elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GMPS purchase

MBS282332 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GMP synthase (GMPS) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GMPS. The ELISA analytical biochemical technique of the MBS282332 kit is based on GMPS antibody-GMPS antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GMPS antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GMPS. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GMPS. It may not necessarily be applicable to this product.

NCBI GI #

4504035

NCBI GeneID

8833

NCBI Accession #

NP_003866.1

UniProt Primary Accession #

P49915

UniProt Secondary Accession #

A8K639; B4DXV7; F8W720

UniProt Related Accession #

P49915

GenBank Nucleotide #

NM_003875.2

NCBI Official Full Name

GMP synthase

NCBI Official Synonym Full Names

guanine monophosphate synthase

NCBI Official Symbol

GMPS

NCBI Official Synonym Symbols

GATD7

UniProt Protein Name

GMP synthase [glutamine-hydrolyzing]

UniProt Synonym Protein Names

GMP synthetase; Glutamine amidotransferase

Protein Family

GMP synthase

UniProt Gene Name

GMPS

NCBI Protein Information

GMP synthase [glutamine-hydrolyzing]

NCBI Summary for GMPS

In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008]

UniProt Comments for GMPS

GMPS: Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division. A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL.

Protein type: EC 6.3.5.2; Ligase; Nucleotide Metabolism - purine; Oncoprotein; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 3q25.31

Cellular Component: cytosol

Molecular Function: ATP binding; GMP synthase (glutamine-hydrolyzing) activity; GMP synthase activity; pyrophosphatase activity

Biological Process: glutamine metabolic process; GMP biosynthetic process; purine nucleobase biosynthetic process; purine ribonucleoside monophosphate biosynthetic process

Disease: Leukemia, Acute Myeloid

Research Articles on GMPS

1. Three genes-ATP6V1G1 in 9q32, GMPS in 3q25.31, and TBX5 in 12q24.21-exhibited concomitant hypermethylation and decreased expression. The i(12p)-positive cells displayed global hypomethylation of gene-poor regions on 12p, a footprint previously associated with constitutional and acquired gains of whole chromosomes as well as with X-chromosome inactivation in females

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GMPS elisa kit

Reference Product	PubMed Publications
USP7 elisa kit	>5 publications with GMPS and USP7
HPRT1 elisa kit	>1 publications with GMPS and HPRT1
IMPDH2 elisa kit	>1 publications with GMPS and IMPDH2
IMPDH1 elisa kit	>1 publications with GMPS and IMPDH1

Pathways associated with GMPS elisa kit

Products by Pathway	Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Drug Metabolism - Other Enzymes Pathway antibodies	Drug Metabolism - Other Enzymes Pathway Diagram
Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway antibodies	Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway Diagram
Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway antibodies	Guanine Ribonucleotide Biosynthesis IMP => GDP,GTP Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram

Diseases associated with GMPS elisa kit

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>26 publications with GMPS and Heart Diseases
Leukemia, Myeloid, Acute Antibodies	>15 publications with GMPS and Leukemia, Myeloid, Acute
Disease Models, Animal Antibodies	>11 publications with GMPS and Disease Models, Animal
Cell Transformation, Neoplastic Antibodies	>8 publications with GMPS and Cell Transformation, Neoplastic
Anemia Antibodies	>6 publications with GMPS and Anemia
Carcinoma Antibodies	>3 publications with GMPS and Carcinoma
Adenocarcinoma Antibodies	>3 publications with GMPS and Adenocarcinoma
Neoplasms, Experimental Antibodies	>2 publications with GMPS and Neoplasms, Experimental
Precancerous Conditions Antibodies	>2 publications with GMPS and Precancerous Conditions
Kidney Diseases Antibodies	>2 publications with GMPS and Kidney Diseases

Organs/Tissues associated with GMPS elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>74 publications with GMPS and Blood
Bone Marrow Antibodies	>34 publications with GMPS and Bone Marrow
Heart Antibodies	>21 publications with GMPS and Heart
Vascular Antibodies	>21 publications with GMPS and Vascular
Muscle Antibodies	>13 publications with GMPS and Muscle
Liver Antibodies	>9 publications with GMPS and Liver
Mouth Antibodies	>8 publications with GMPS and Mouth
Spleen Antibodies	>7 publications with GMPS and Spleen
Connective Tissue Antibodies	>5 publications with GMPS and Connective Tissue
Brain Antibodies	>5 publications with GMPS and Brain