Putative Methyltransferase NSUN5 (NSUN5) ELISA Kit for Human

NSUN5 encodes a member of the evolutionarily conserved NOL1/NOP2/Sun domain family. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. WBSCR20 encodes a novel protein expressed in skeletal muscle with similarity to p120 (NOL1), a 120-kDa proliferation-associated nucleolar antigen, a member of an evolutionarily conserved protein family. A highly similar putative gene, WBSCR20B, flanks the WBS deletion at the telomeric side. Hemizygous deletion of either of the novel genes might contribute to the growth retardation, the myopathy or the premature aging effects in the pathogenesis of WBS.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NSUN5 in samples. An antibody specific for NSUN5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNSUN5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NSUN5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NSUN5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of NSUN5 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS282348 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Putative methyltransferase NSUN5, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NSUN5. The ELISA analytical biochemical technique of the MBS282348 kit is based on NSUN5 antibody-NSUN5 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NSUN5 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NSUN5. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).