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Deleted in Malignant Brain Tumors 1 (DMBT1) ELISA Kit for Pig

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282406

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DMBT1 in Pig serum, plasma, tissue homogenates. An antibody specific for DMBT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Deleted in malignant brain tumors 1 protein (DMBT1), ELISA Kit
Full Product Name
Pig Deleted in malignant brain tumors 1 protein (DMBT1) ELISA Kit
Product Gene Name
Species Reactivity
Pig
Assay Type
Quantitative Sandwich
Samples
Malignant brain tumors 1 protein (DMBT1) concentration in serum, plasma, tissue homogenates and other biological fluids
Detection Range
31.25 pg/mL - 2000 pg/mL
Sensitivity
15.6 pg/mL (mean of 6 independent assays)
Specificity
This assay has high sensitivity and excellent specificity for detection of Pig DMBT1. No significant cross-reactivity or interference between Pig DMBT1 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Molecular Weight
147,857 Da
3D Structure
ModBase 3D Structure for Q4A3R3
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DMBT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DMBT1 purchase

MBS282406 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Deleted in malignant brain tumors 1 protein (DMBT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DMBT1. The ELISA analytical biochemical technique of the MBS282406 kit is based on DMBT1 antibody-DMBT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DMBT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DMBT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DMBT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
deleted in malignant brain tumors 1 protein
NCBI Official Symbol
DMBT1
UniProt Protein Name
Deleted in malignant brain tumors 1 protein
UniProt Synonym Protein Names
Hensin
UniProt Gene Name
DMBT1
NCBI Protein Information
deleted in malignant brain tumors 1 protein

UniProt Comments for DMBT1

May play roles in mucosal defense system and cellular immune defense. May play a role in liver regeneration. May be an important factor in fate decision and differentiation of transit-amplifying ductular (oval) cells within the hepatic lineage. May function as a binding protein in saliva for the regulation of taste sensation. May play a role as an opsonin receptor for SFTPD and SPAR in macrophage tissues throughout the body, including epithelial cells lining the gastrointestinal tract. Required for terminal differentiation of columnar epithelial cells during early embryogenesis. Displays a broad calcium-dependent binding spectrum against both Gram-positive and Gram-negative bacteria, suggesting a role in defense against bacterial pathogens. Binds to a range of poly-sulfated and poly-phosphorylated ligands which may explain its broad bacterial-binding specificity. Inhibits cytoinvasion of S.enterica. Associates with the actin cytoskeleton and is involved in its remodeling during regulated exocytosis. Interacts with pancreatic zymogens in a pH-dependent manner and may act as a Golgi cargo receptor in the regulated secretory pathway of the pancreatic acinar cell ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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