Glycine N-Methyltransferase (GNMT) ELISA Kit for Human

Name: Human GNMT ELISA Kit; Glycine N-Methyltransferase; MyBioSource
Brand: MyBioSource
SKU: MBS282407
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282407

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GNMT in samples. An antibody specific for GNMT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGNMT present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Glycine N-methyltransferase (GNMT), ELISA Kit

Full Product Name

Human Glycine N-methyltransferase (GNMT) ELISA Kit

Product Gene Name

GNMT elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human GNMT. No significant cross-reactivity or interference between Human GNMT and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

32,742 Da

OMIM

AF101477 mRNA

3D Structure

ModBase 3D Structure for Q14749

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GNMT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GNMT purchase

MBS282407 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glycine N-methyltransferase (GNMT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GNMT. The ELISA analytical biochemical technique of the MBS282407 kit is based on GNMT antibody-GNMT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GNMT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GNMT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GNMT. It may not necessarily be applicable to this product.

NCBI GI #

974141082

NCBI GeneID

27232

NCBI Accession #

NP_001305794.1

UniProt Primary Accession #

Q14749

UniProt Secondary Accession #

Q5T8W2; Q9NNZ1; Q9NS24

UniProt Related Accession #

Q14749

GenBank Nucleotide #

NM_001318865.1

NCBI Official Full Name

glycine N-methyltransferase isoform 2

NCBI Official Synonym Full Names

glycine N-methyltransferase

NCBI Official Symbol

GNMT

NCBI Official Synonym Symbols

HEL-S-182mP

UniProt Protein Name

Glycine N-methyltransferase

Protein Family

Glycine N-methyltransferase

UniProt Gene Name

GNMT

NCBI Protein Information

glycine N-methyltransferase

NCBI Summary for GNMT

The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. This protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). Alternative splicing results in multiple transcript variants. Naturally occurring readthrough transcription occurs between the upstream CNPY3 (canopy FGF signaling regulator 3) gene and this gene and is represented with GeneID:107080644. [provided by RefSeq, Jan 2016]

UniProt Comments for GNMT

GNMT: Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Defects in GNMT are the cause of glycine N- methyltransferase deficiency (GNMT deficiency); also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases. Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; EC 2.1.1.20; Methyltransferase

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: cytosol

Molecular Function: folic acid binding; glycine binding; glycine N-methyltransferase activity; protein binding

Biological Process: cellular protein modification process; glycogen metabolic process; methionine metabolic process; methylation; one-carbon metabolic process; protein homotetramerization; regulation of gluconeogenesis; S-adenosylmethionine metabolic process

Disease: Glycine N-methyltransferase Deficiency

Research Articles on GNMT

1. Elevated GNMT protein expression is associated with pathogenesis of hepatocellular carcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with GNMT elisa kit

Products by Pathway	Pathway Diagram
Glycine, Serine And Threonine Metabolism Pathway antibodies	Glycine, Serine And Threonine Metabolism Pathway Diagram
Glycine, Serine And Threonine Metabolism Pathway antibodies	Glycine, Serine And Threonine Metabolism Pathway Diagram
Glyoxylate Metabolism And Glycine Degradation Pathway antibodies	Glyoxylate Metabolism And Glycine Degradation Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Metabolism Of Ingested SeMet, Sec, MeSec Into H2Se Pathway antibodies	Metabolism Of Ingested SeMet, Sec, MeSec Into H2Se Pathway Diagram
Selenoamino Acid Metabolism Pathway antibodies	Selenoamino Acid Metabolism Pathway Diagram

Diseases associated with GNMT elisa kit

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>53 publications with GNMT and Liver Diseases
Carcinoma, Hepatocellular Antibodies	>41 publications with GNMT and Carcinoma, Hepatocellular
Liver Neoplasms Antibodies	>30 publications with GNMT and Liver Neoplasms
Adenocarcinoma Antibodies	>27 publications with GNMT and Adenocarcinoma
Disease Models, Animal Antibodies	>24 publications with GNMT and Disease Models, Animal
Fatty Liver Antibodies	>17 publications with GNMT and Fatty Liver
Hepatitis Antibodies	>13 publications with GNMT and Hepatitis
Hyperhomocysteinemia Antibodies	>9 publications with GNMT and Hyperhomocysteinemia
Hepatitis, Chronic Antibodies	>9 publications with GNMT and Hepatitis, Chronic
Inflammation Antibodies	>8 publications with GNMT and Inflammation

Organs/Tissues associated with GNMT elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>124 publications with GNMT and Liver
Blood Antibodies	>31 publications with GNMT and Blood
Prostate Antibodies	>12 publications with GNMT and Prostate
Kidney Antibodies	>7 publications with GNMT and Kidney
Pancreas Antibodies	>5 publications with GNMT and Pancreas
Lung Antibodies	>3 publications with GNMT and Lung