Phosphatidylinositol-Glycan-Specific Phospholipase D (GPLD1) ELISA Kit for Human

Name: Human GPLD1 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS282459
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282459

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GPLD1 in samples. An antibody specific for GPLD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGPLD1 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Phosphatidylinositol-glycan-specific phospholipase D (GPLD1), ELISA Kit

Full Product Name

Human Phosphatidylinositol-glycan-specific phospholipase D (GPLD1) ELISA Kit

Product Gene Name

GPLD1 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human GPLD1. No significant cross-reactivity or interference between Human GPLD1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

19,865 Da

OMIM

L11701 mRNA

3D Structure

ModBase 3D Structure for P80108

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of GPLD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GPLD1 purchase

MBS282459 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Phosphatidylinositol-glycan-specific phospholipase D (GPLD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GPLD1. The ELISA analytical biochemical technique of the MBS282459 kit is based on GPLD1 antibody-GPLD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GPLD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GPLD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GPLD1. It may not necessarily be applicable to this product.

NCBI GI #

29171717

NCBI GeneID

2822

NCBI Accession #

NP_001494.2

UniProt Primary Accession #

P80108

UniProt Secondary Accession #

Q15127; Q15128; Q2M2F2; Q5T3Y0; Q7Z6T8; Q8TCV0; Q8WW82; Q96ID6; Q9H167; Q9H4M1; Q9UJC9

UniProt Related Accession #

P80108

GenBank Nucleotide #

NM_001503.3

NCBI Official Full Name

phosphatidylinositol-glycan-specific phospholipase D

NCBI Official Synonym Full Names

glycosylphosphatidylinositol specific phospholipase D1

NCBI Official Symbol

GPLD1

NCBI Official Synonym Symbols

PLD; GPIPLD; PIGPLD; GPIPLDM; PIGPLD1

UniProt Protein Name

Phosphatidylinositol-glycan-specific phospholipase D

UniProt Synonym Protein Names

Glycoprotein phospholipase D; Glycosyl-phosphatidylinositol-specific phospholipase D; GPI-PLD; GPI-specific phospholipase D

Protein Family

Phosphatidylinositol-glycan-specific phospholipase

UniProt Gene Name

GPLD1

UniProt Synonym Gene Names

PIGPLD1; PI-G PLD; GPI-PLD; GPI-specific phospholipase D

NCBI Protein Information

phosphatidylinositol-glycan-specific phospholipase D

NCBI Summary for GPLD1

Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008]

UniProt Comments for GPLD1

GPLD1: This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. Belongs to the GPLD1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.4.50; Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Phospholipase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytoplasm; extracellular exosome; extracellular region; extracellular space; intracellular; intracellular membrane-bound organelle; lysosomal membrane; proteinaceous extracellular matrix

Molecular Function: glycosylphosphatidylinositol phospholipase D activity; phospholipase D activity; sodium channel regulator activity

Biological Process: C-terminal protein lipidation; cell migration during sprouting angiogenesis; cellular response to calcium ion; cellular response to cholesterol; cellular response to insulin stimulus; cellular response to pH; cellular response to triglyceride; chondrocyte differentiation; complement receptor mediated signaling pathway; GPI anchor release; insulin receptor signaling pathway; negative regulation of cell proliferation; negative regulation of triglyceride catabolic process; ossification; phosphatidylcholine metabolic process; positive regulation of alkaline phosphatase activity; positive regulation of apoptosis; positive regulation of cytolysis; positive regulation of endothelial cell migration; positive regulation of glucose metabolic process; positive regulation of high-density lipoprotein particle clearance; positive regulation of membrane protein ectodomain proteolysis; positive regulation of secretion; positive regulation of triglyceride biosynthetic process; regulation of cellular response to insulin stimulus; response to glucose stimulus

Research Articles on GPLD1

1. Study discovered novel and independent associations of prediabetes and related traits with MASP1, and some evidence for associations with THBS1, GPLD1 and ApoA-IV, suggesting a role for these proteins in the pathophysiology of type 2 diabetes.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with GPLD1 elisa kit

Products by Pathway	Pathway Diagram
Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway antibodies	Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway Diagram
Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway antibodies	Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway Diagram

Diseases associated with GPLD1 elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>2 publications with GPLD1 and Inflammation
Heart Diseases Antibodies	>1 publications with GPLD1 and Heart Diseases
Nervous System Diseases Antibodies	>1 publications with GPLD1 and Nervous System Diseases
Liver Diseases Antibodies	>1 publications with GPLD1 and Liver Diseases

Organs/Tissues associated with GPLD1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5 publications with GPLD1 and Blood
Liver Antibodies	>4 publications with GPLD1 and Liver
Kidney Antibodies	>1 publications with GPLD1 and Kidney
Pancreas Antibodies	>1 publications with GPLD1 and Pancreas
Embryonic Tissue Antibodies	>1 publications with GPLD1 and Embryonic Tissue
Lung Antibodies	>1 publications with GPLD1 and Lung