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5-Hydroxytryptamine Receptor 7 (HTR7) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282566

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HEPC25 in Human serum, plasma. An antibody specific for HEPC25 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HEPC25 present… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
PDF
Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
5-hydroxytryptamine receptor 7 (HTR7), ELISA Kit
Full Product Name
Rat 5-hydroxytryptamine receptor 7 (HTR7) ELISA Kit
Product Gene Name
HTR7 elisa kit
Species Reactivity
Rat
Assay Type
Sandwich
Samples
Serum, Plasma And Other Biological Fluids
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.78 ng/mL (mean of 6 independent assays).
Specificity
This assay has high sensitivity and excellent specificity for detection of Human HEPC25. No significant cross-reactivity or interference between Human HEPC25 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Molecular Weight
48,428 Da
3D Structure
ModBase 3D Structure for P32305
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of HTR7 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HTR7 purchase

MBS282566 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 5-hydroxytryptamine receptor 7 (HTR7) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HTR7. The ELISA analytical biochemical technique of the MBS282566 kit is based on HTR7 antibody-HTR7 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTR7 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTR7. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HTR7. It may not necessarily be applicable to this product.

NCBI GI #
12621102
NCBI GeneID
65032
NCBI Accession #
NP_075227.1
UniProt Primary Accession #
P32305
UniProt Secondary Accession #
P97936
UniProt Related Accession #
P32305
GenBank Nucleotide #
NM_022938.2
NCBI Official Full Name
5-hydroxytryptamine receptor 7
NCBI Official Synonym Full Names
5-hydroxytryptamine (serotonin) receptor 7, adenylate cyclase-coupled
NCBI Official Symbol
Htr7
NCBI Official Synonym Symbols
5Ht7
UniProt Protein Name
5-hydroxytryptamine receptor 7
UniProt Synonym Protein Names
5-HT-X; GPRFO; Serotonin receptor 7
Protein Family
5-hydroxytryptamine receptor
UniProt Gene Name
Htr7
UniProt Synonym Gene Names
5-HT-7; 5-HT7
NCBI Protein Information
5-hydroxytryptamine receptor 7

NCBI Summary for HTR7

receptor for 5-hydroxytryptamine; involved in modulatory action on cerebellar function [RGD, Feb 2006]

UniProt Comments for HTR7

5-HT(7): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 1q53

Cellular Component: axon terminus; cell soma; dendrite; integral component of membrane; plasma membrane; rough endoplasmic reticulum; synaptic vesicle

Molecular Function: G-protein coupled serotonin receptor activity; neurotransmitter receptor activity; serotonin binding

Biological Process: adenylate cyclase-activating serotonin receptor signaling pathway; adenylate cyclase-inhibiting serotonin receptor signaling pathway; behavioral response to pain; circadian rhythm; detection of mechanical stimulus involved in sensory perception of pain; G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; memory; micturition; negative regulation of circadian sleep/wake cycle, REM sleep; negative regulation of serotonin secretion; response to corticosterone stimulus; response to electrical stimulus; serotonin receptor signaling pathway; smooth muscle contraction; synaptic transmission; urinary bladder smooth muscle contraction; vasoconstriction
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Research Articles on HTR7

1. Our data provide clear confirmation of the fact that recruitment of descending 5-HT neurons is required for voluntary locomotion in rodents, and that activation of 5-HT7 receptors is necessary for this effect.In addition, our results show that recruitment of descending 5-HT neurons acting through spinal 5-HT7 receptors is necessary for fictive locomotion evoked from the mesencephalic locomotor region.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with HTR7 elisa kit

 Products by Pathway  Pathway Diagram
 Amine Ligand-binding Receptors Pathway antibodies  Amine Ligand-binding Receptors Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Calcium Signaling Pathway antibodies  Calcium Signaling Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class A Rhodopsin-like Pathway antibodies  GPCRs, Class A Rhodopsin-like Pathway Diagram
 GPCRs, Other Pathway antibodies  GPCRs, Other Pathway Diagram
 Monoamine GPCRs Pathway antibodies  Monoamine GPCRs Pathway Diagram

Diseases associated with HTR7 elisa kit

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >13 publications with HTR7 and Mental Disorders
 Nervous System Diseases Antibodies  >5 publications with HTR7 and Nervous System Diseases
 Schizophrenia Antibodies  >5 publications with HTR7 and Schizophrenia
 Inflammation Antibodies  >4 publications with HTR7 and Inflammation
 Disease Models, Animal Antibodies  >3 publications with HTR7 and Disease Models, Animal
 Brain Diseases Antibodies  >3 publications with HTR7 and Brain Diseases
 Muscular Diseases Antibodies  >2 publications with HTR7 and Muscular Diseases
 Mood Disorders Antibodies  >2 publications with HTR7 and Mood Disorders
 Pain Antibodies  >2 publications with HTR7 and Pain
 Drug Toxicity Antibodies  >1 publications with HTR7 and Drug Toxicity

Organs/Tissues associated with HTR7 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7 publications with HTR7 and Brain
 Nerve Antibodies  >3 publications with HTR7 and Nerve
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