Protein Tob1 (TOB1) ELISA Kit for Human

Name: Human TOB1 ELISA Kit; Protein Tob1; MyBioSource
Brand: MyBioSource
SKU: MBS282569
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282569

Intended Uses: For the quantitative detection of Human Protein Tob1 (TOB1) concentration in serum, plasma and other biological fluids.Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TOB1 in Human serum, plasma. An antibody… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Protein Tob1, ELISA Kit

Full Product Name

Human Protein Tob1 (TOB1) ELISA Kit

Product Gene Name

TOB1 elisa kit

Species Reactivity

Human

Samples

serum, plasma and other biological fluids

Detection Range

62.5 pg/mL - 4000 pg/mL

Sensitivity

31.25 pg/mL (mean of 6 independent assays).

Specificity

This assay has high sensitivity and excellent specificity for detection of Human TOB1. No significant cross-reactivity or interference between Human TOB1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

38,155 Da

OMIM

D38305 mRNA

3D Structure

ModBase 3D Structure for P50616

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of TOB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TOB1 purchase

MBS282569 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Tob1, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TOB1. The ELISA analytical biochemical technique of the MBS282569 kit is based on TOB1 antibody-TOB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TOB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TOB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TOB1. It may not necessarily be applicable to this product.

NCBI GI #

345197216

NCBI GeneID

10140

NCBI Accession #

NP_001230806.1

UniProt Primary Accession #

P50616

UniProt Secondary Accession #

Q4KMQ0; B2R9T0; D3DTY3

UniProt Related Accession #

P50616

GenBank Nucleotide #

NM_001243877.1

NCBI Official Full Name

protein Tob1 isoform 1

NCBI Official Synonym Full Names

transducer of ERBB2, 1

NCBI Official Symbol

TOB1

NCBI Official Synonym Symbols

TOB; TROB; APRO5; APRO6; PIG49; TROB1

UniProt Protein Name

Protein Tob1

UniProt Synonym Protein Names

Transducer of erbB-2 1

Protein Family

Protein

UniProt Gene Name

TOB1

UniProt Synonym Gene Names

TOB; TROB1

NCBI Protein Information

protein Tob1

NCBI Summary for TOB1

This gene encodes a member of the transducer of erbB-2 /B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The encoded protein may function as a tumor suppressor. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

UniProt Comments for TOB1

TOB1: Anti-proliferative protein that interacts with the erbB- 2 receptor tyrosine kinase. May physically and/or functionally interact with protein-tyrosine kinase receptors. Belongs to the BTG family.

Protein type: Adaptor/scaffold; Cell cycle regulation

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: CCR4-NOT complex; cytoplasm; nucleus

Molecular Function: protein binding; receptor tyrosine kinase binding; SH3/SH2 adaptor activity; SMAD binding; transcription corepressor activity

Biological Process: BMP signaling pathway; negative regulation of BMP signaling pathway; negative regulation of cell proliferation; negative regulation of osteoblast differentiation; negative regulation of translation; positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; positive regulation of signal transduction; SMAD protein import into nucleus

Research Articles on TOB1

1. MiR-590 targets Tob1 that is significantly decreased in patients with multiple sclerosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TOB1 elisa kit

Reference Product	PubMed Publications
ERBB2 elisa kit	>21 publications with TOB1 and ERBB2
CNOT7 elisa kit	>5 publications with TOB1 and CNOT7
SMAD4 elisa kit	>3 publications with TOB1 and SMAD4
SMAD2 elisa kit	>3 publications with TOB1 and SMAD2
SMAD1 elisa kit	>3 publications with TOB1 and SMAD1
MAPK1 elisa kit	>3 publications with TOB1 and MAPK1
SMAD3 elisa kit	>2 publications with TOB1 and SMAD3
SMAD6 elisa kit	>1 publications with TOB1 and SMAD6
SMAD5 elisa kit	>1 publications with TOB1 and SMAD5
MARCKS elisa kit	>1 publications with TOB1 and MARCKS

Pathways associated with TOB1 elisa kit

Products by Pathway	Pathway Diagram
BMP Signalling And Regulation Pathway antibodies	BMP Signalling And Regulation Pathway Diagram
Diurnally Regulated Genes With Circadian Orthologs Pathway antibodies	Diurnally Regulated Genes With Circadian Orthologs Pathway Diagram
RNA Degradation Pathway antibodies	RNA Degradation Pathway Diagram
RNA Degradation Pathway antibodies	RNA Degradation Pathway Diagram

Diseases associated with TOB1 elisa kit

Disease Name	Pubmed Publications
Neoplasms Antibodies	>25 publications with TOB1 and Neoplasms
Carcinoma Antibodies	>14 publications with TOB1 and Carcinoma
Breast Neoplasms Antibodies	>8 publications with TOB1 and Breast Neoplasms
Lung Neoplasms Antibodies	>5 publications with TOB1 and Lung Neoplasms
Adenocarcinoma Antibodies	>5 publications with TOB1 and Adenocarcinoma
Lung Diseases Antibodies	>4 publications with TOB1 and Lung Diseases
Kidney Diseases Antibodies	>2 publications with TOB1 and Kidney Diseases
Carcinoma, Squamous Cell Antibodies	>2 publications with TOB1 and Carcinoma, Squamous Cell
Liver Neoplasms, Experimental Antibodies	>1 publications with TOB1 and Liver Neoplasms, Experimental
Carcinoma, Hepatocellular Antibodies	>1 publications with TOB1 and Carcinoma, Hepatocellular

Organs/Tissues associated with TOB1 elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>11 publications with TOB1 and Lung
Kidney Antibodies	>5 publications with TOB1 and Kidney
Embryonic Tissue Antibodies	>4 publications with TOB1 and Embryonic Tissue
Liver Antibodies	>2 publications with TOB1 and Liver
Thyroid Antibodies	>2 publications with TOB1 and Thyroid
Lymph Node Antibodies	>2 publications with TOB1 and Lymph Node
Intestine Antibodies	>2 publications with TOB1 and Intestine
Prostate Antibodies	>2 publications with TOB1 and Prostate
Stomach Antibodies	>2 publications with TOB1 and Stomach
Skin Antibodies	>1 publications with TOB1 and Skin