Probable ATP-Dependent RNA Helicase DDX27 (DDX27) ELISA Kit for Human

Name: Human DDX27 ELISA Kit; Probable ATP-Dependent RNA Helicase DDX27; MyBioSource
Brand: MyBioSource
SKU: MBS282604
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282604

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DDX27 in samples. An antibody specific for DDX27 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDDX27 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Probable ATP-dependent RNA helicase DDX27, ELISA Kit

Full Product Name

Human Probable ATP-dependent RNA helicase DDX27 (DDX27) ELISA Kit

Product Gene Name

DDX27 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human DDX27. No significant cross-reactivity or interference between Human DDX27 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

89,835 Da

OMIM

AY044431 mRNA

3D Structure

ModBase 3D Structure for Q96GQ7

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DDX27 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DDX27 purchase

MBS282604 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable ATP-dependent RNA helicase DDX27, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DDX27. The ELISA analytical biochemical technique of the MBS282604 kit is based on DDX27 antibody-DDX27 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DDX27 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DDX27. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DDX27. It may not necessarily be applicable to this product.

NCBI GI #

224593278

NCBI GeneID

55661

NCBI Accession #

NP_060365.7

UniProt Primary Accession #

Q96GQ7

UniProt Secondary Accession #

Q5VXM7; Q8WYG4; Q969N7; Q96F57; Q96L97; Q9BWY9; Q9BXF0; Q9H990; Q9NWU3; A0AVB6; B7ZLY1

UniProt Related Accession #

Q96GQ7

GenBank Nucleotide #

NM_017895.7

NCBI Official Full Name

probable ATP-dependent RNA helicase DDX27 isoform 2

NCBI Official Synonym Full Names

DEAD-box helicase 27

NCBI Official Symbol

DDX27

NCBI Official Synonym Symbols

DRS1; RHLP; Drs1p; PP3241; HSPC259; dJ686N3.1

UniProt Protein Name

Probable ATP-dependent RNA helicase DDX27

UniProt Synonym Protein Names

DEAD box protein 27

Protein Family

Probable ATP-dependent RNA helicase

UniProt Gene Name

DDX27

NCBI Protein Information

probable ATP-dependent RNA helicase DDX27

NCBI Summary for DDX27

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein involved in the processing of 5.8S and 28S ribosomal RNAs. More specifically, the encoded protein localizes to the nucleolus, where it interacts with the PeBoW complex to ensure proper 3' end formation of 47S rRNA. [provided by RefSeq, Jan 2017]

UniProt Comments for DDX27

DDX27: Probable ATP-dependent RNA helicase. Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.

Protein type: EC 3.6.4.13; Helicase; RNA-binding

Chromosomal Location of Human Ortholog: 20q13.13

Cellular Component: chromosome; cytoplasm; nucleolus

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; protein binding; RNA binding

Biological Process: RNA secondary structure unwinding; rRNA processing

Research Articles on DDX27

1. The DDX27 can interact specifically with the Pes1 and Bop1 but fulfils critical function(s) for proper 3' end formation of 47S rRNA independently of the PeBoW-complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with DDX27 elisa kit

Disease Name	Pubmed Publications
Chromosome Aberrations Antibodies	>1 publications with DDX27 and Chromosome Aberrations