Plasma Membrane Calcium-Transporting Atpase 2 (ATP2B2) ELISA Kit for Human

Name: Human ATP2B2 ELISA Kit; Plasma Membrane Calcium-Transporting Atpase 2; MyBioSource
Brand: MyBioSource
SKU: MBS282696
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282696

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ATP2B2 in samples. An antibody specific for ATP2B2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyATP2B2 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Plasma membrane calcium-transporting ATPase 2 (ATP2B2), ELISA Kit

Full Product Name

Human Plasma membrane calcium-transporting ATPase 2 (ATP2B2) ELISA Kit

Product Gene Name

ATP2B2 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human ATP2B2. No significant cross-reactivity or interference between Human ATP2B2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

134,040 Da

OMIM

M97260 mRNA

3D Structure

ModBase 3D Structure for Q01814

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of ATP2B2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATP2B2 purchase

MBS282696 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Plasma membrane calcium-transporting ATPase 2 (ATP2B2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATP2B2. The ELISA analytical biochemical technique of the MBS282696 kit is based on ATP2B2 antibody-ATP2B2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATP2B2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATP2B2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ATP2B2. It may not necessarily be applicable to this product.

NCBI GI #

48255951

NCBI GeneID

491

NCBI Accession #

NP_001001331.1

UniProt Primary Accession #

Q01814

UniProt Secondary Accession #

O00766; Q12994; Q16818

UniProt Related Accession #

Q01814

GenBank Nucleotide #

NM_001001331.3

NCBI Official Full Name

plasma membrane calcium-transporting ATPase 2 isoform 1

NCBI Official Synonym Full Names

ATPase plasma membrane Ca2+ transporting 2

NCBI Official Symbol

ATP2B2

NCBI Official Synonym Symbols

PMCA2; PMCA2a; PMCA2i

UniProt Protein Name

Plasma membrane calcium-transporting ATPase 2

UniProt Synonym Protein Names

Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2

Protein Family

Plasma membrane calcium-transporting ATPase

UniProt Gene Name

ATP2B2

UniProt Synonym Gene Names

PMCA2; PMCA2

NCBI Protein Information

plasma membrane calcium-transporting ATPase 2

NCBI Summary for ATP2B2

The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

UniProt Comments for ATP2B2

ATP2B2: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; EC 3.6.3.8; Endoplasmic reticulum; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ion channel

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cell junction; cytoplasm; integral component of plasma membrane; intracellular membrane-bound organelle; plasma membrane; synapse

Molecular Function: ATP binding; calcium-transporting ATPase activity; calmodulin binding; metal ion binding; PDZ domain binding; protein binding

Biological Process: calcium ion transport; neuron differentiation; regulation of cytosolic calcium ion concentration; sensory perception of sound; transport

Disease: Deafness, Autosomal Recessive 12

Research Articles on ATP2B2

1. Cytoskeleton dynamics regulates plasma membrane PMCA2 activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ATP2B2 elisa kit

Products by Pathway	Pathway Diagram
Adrenergic Signaling In Cardiomyocytes Pathway antibodies	Adrenergic Signaling In Cardiomyocytes Pathway Diagram
Adrenergic Signaling In Cardiomyocytes Pathway antibodies	Adrenergic Signaling In Cardiomyocytes Pathway Diagram
Calcium Regulation In The Cardiac Cell Pathway antibodies	Calcium Regulation In The Cardiac Cell Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Calcium Signaling Pathway antibodies	Calcium Signaling Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Ion Channel Transport Pathway antibodies	Ion Channel Transport Pathway Diagram
Ion Transport By P-type ATPases Pathway antibodies	Ion Transport By P-type ATPases Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram
Pancreatic Secretion Pathway antibodies	Pancreatic Secretion Pathway Diagram

Diseases associated with ATP2B2 elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>31 publications with ATP2B2 and Nervous System Diseases
Disease Models, Animal Antibodies	>9 publications with ATP2B2 and Disease Models, Animal
Death Antibodies	>8 publications with ATP2B2 and Death
Breast Neoplasms Antibodies	>4 publications with ATP2B2 and Breast Neoplasms
Heart Diseases Antibodies	>4 publications with ATP2B2 and Heart Diseases
Inflammation Antibodies	>3 publications with ATP2B2 and Inflammation
Nerve Degeneration Antibodies	>2 publications with ATP2B2 and Nerve Degeneration
Hypertrophy Antibodies	>2 publications with ATP2B2 and Hypertrophy
Prenatal Injuries Antibodies	>1 publications with ATP2B2 and Prenatal Injuries
Infertility, Male Antibodies	>1 publications with ATP2B2 and Infertility, Male

Organs/Tissues associated with ATP2B2 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>31 publications with ATP2B2 and Brain
Eye Antibodies	>8 publications with ATP2B2 and Eye
Blood Antibodies	>5 publications with ATP2B2 and Blood
Nerve Antibodies	>4 publications with ATP2B2 and Nerve
Muscle Antibodies	>4 publications with ATP2B2 and Muscle
Kidney Antibodies	>2 publications with ATP2B2 and Kidney
Ovary Antibodies	>1 publications with ATP2B2 and Ovary
Intestine Antibodies	>1 publications with ATP2B2 and Intestine