Probable Methyltransferase (TARBP1) ELISA Kit for Human

Name: Human TARBP1 ELISA Kit; Probable Methyltransferase; MyBioSource
Brand: MyBioSource
SKU: MBS282706
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282706

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate TARBP1 in samples. An antibody specific for TARBP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTARBP1 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Probable methyltransferase (TARBP1), ELISA Kit

Full Product Name

Human Probable methyltransferase (TARBP1) ELISA Kit

Product Gene Name

TARBP1 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human TARBP1. No significant cross-reactivity or interference between Human TARBP1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

181,675 Da

OMIM

U38847 mRNA

3D Structure

ModBase 3D Structure for Q13395

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of TARBP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TARBP1 purchase

MBS282706 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable methyltransferase (TARBP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TARBP1. The ELISA analytical biochemical technique of the MBS282706 kit is based on TARBP1 antibody-TARBP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TARBP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TARBP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TARBP1. It may not necessarily be applicable to this product.

NCBI GI #

110825988

NCBI GeneID

6894

NCBI Accession #

NP_005637.3

UniProt Primary Accession #

Q13395

UniProt Secondary Accession #

Q9H581

UniProt Related Accession #

Q13395

GenBank Nucleotide #

NM_005646.3

NCBI Official Full Name

probable methyltransferase TARBP1

NCBI Official Synonym Full Names

TAR (HIV-1) RNA binding protein 1

NCBI Official Symbol

TARBP1

NCBI Official Synonym Symbols

TRM3; TRMT3; TRP185; TRP-185

UniProt Protein Name

Probable methyltransferase TARBP1

UniProt Synonym Protein Names

TAR RNA-binding protein 1; TAR RNA-binding protein of 185 kDa; TRP-185

Protein Family

Probable methyltransferase

UniProt Gene Name

TARBP1

UniProt Synonym Gene Names

TRM3; TRP185; TRP-185

NCBI Protein Information

probable methyltransferase TARBP1

NCBI Summary for TARBP1

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. This element forms a stable stem-loop structure and can be bound by either the protein encoded by this gene or by RNA polymerase II. This protein may act to disengage RNA polymerase II from TAR during transcriptional elongation. Alternatively spliced transcripts of this gene may exist, but their full-length natures have not been determined. [provided by RefSeq, Jul 2008]

UniProt Comments for TARBP1

TARBP1: Probable S-adenosyl-L-methionine-dependent methyltransferase which methylates RNA molecules such as tRNAs. In case of infection by HIV-1, it binds to the loop region of TAR RNA, a region also bound by RNA polymerase II. Binding of TARBP1 and RNA polymerase II to HIV-1 TAR RNA is mutually exclusive, suggesting that TARBP1 may function alone or in conjunction with HIV-1 Tat to disengage RNA polymerase II from HIV-1 TAR RNA. May act by methylating HIV-1 TAR RNA. Belongs to the RNA methyltransferase TrmH family.

Protein type: EC 2.1.1.-; Methyltransferase; RNA-binding

Chromosomal Location of Human Ortholog: 1q42.2

Cellular Component: nucleus

Molecular Function: RNA binding; tRNA (guanine) methyltransferase activity

Biological Process: enzyme-directed rRNA 2'-O-methylation; regulation of transcription from RNA polymerase II promoter; tRNA methylation

Research Articles on TARBP1

1. TARBP1 is up-regulated in hepatocellular carcinoma, and the expression of TARBP1 was associated with the pathological grading and clinical stage.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TARBP1 elisa kit

Reference Product	PubMed Publications
TAT elisa kit	>8 publications with TARBP1 and TAT

Diseases associated with TARBP1 elisa kit

Disease Name	Pubmed Publications
Neoplasm Metastasis Antibodies	>1 publications with TARBP1 and Neoplasm Metastasis
Precancerous Conditions Antibodies	>1 publications with TARBP1 and Precancerous Conditions

Organs/Tissues associated with TARBP1 elisa kit

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>2 publications with TARBP1 and Skin
Bone Marrow Antibodies	>1 publications with TARBP1 and Bone Marrow
Blood Antibodies	>1 publications with TARBP1 and Blood