Y-Box-Binding Protein 2 (YBX2) ELISA Kit for Mouse

YBX2 is a germ-cell specific member of the vertebrate Y box family of nucleic acid-binding proteins . The deduced 364-amino acid protein has a calculated molecular mass of 38.6 kD and is arginine- and proline-rich. It shares 69% and 73% amino acid identity with DBPA (CSDA) and YB1 (NSEP1), respectively, and contains an N-terminal cold-shock domain (CSD), 7 putative phosphorylation sites for casein kinase II, 3 potential phosphorylation sites for protein kinase C, and 2 nuclear localization signals within a B/A island. Northern blot analysis detected a 1.8-kb transcript in human testes only. YBX2 shares 93% and 77% amino acid identity with mouse Ybx2 and Xenopus Frgy2, respectively.The deduced 360-amino acid mouse Msy2 and 282-amino acid Msy2a isoforms have calculated molecular masses of 38 and 31 kD, respectively.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate YBX2 in samples. An antibody specific for YBX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYBX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YBX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YBX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of YBX2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS282712 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Y-box-binding protein 2 (YBX2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing YBX2. The ELISA analytical biochemical technique of the MBS282712 kit is based on YBX2 antibody-YBX2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect YBX2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, YBX2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).