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Wd Repeat-Containing Protein 82 (WDR82) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282778

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate WDR82 in samples. An antibody specific for WDR82 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWDR82 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
WD repeat-containing protein 82 (WDR82), ELISA Kit
Full Product Name
Mouse WD repeat-containing protein 82 (WDR82) ELISA Kit
Product Gene Name
WDR82 elisa kit
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, Plasma, Other biological fluids
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse WDR82. No significant cross-reactivity or interference between Mouse WDR82 and analogues was observed.
Preparation and Storage
Store at 2-8 degree C.
Molecular Weight
35,079 Da
3D Structure
ModBase 3D Structure for Q8BFQ4
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of WDR82 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WDR82 purchase

MBS282778 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the WD repeat-containing protein 82 (WDR82) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WDR82. The ELISA analytical biochemical technique of the MBS282778 kit is based on WDR82 antibody-WDR82 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WDR82 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WDR82. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for WDR82. It may not necessarily be applicable to this product.

NCBI GI #
148223079
NCBI GeneID
77305
NCBI Accession #
NP_084172.1
UniProt Primary Accession #
Q8BFQ4
UniProt Secondary Accession #
Q8K2G5; Q8VEE8
UniProt Related Accession #
Q8BFQ4
GenBank Nucleotide #
NM_029896.1
NCBI Official Full Name
WD repeat-containing protein 82
NCBI Official Synonym Full Names
WD repeat domain containing 82
NCBI Official Symbol
Wdr82
NCBI Official Synonym Symbols
CDW5/WDR82; 9430077D24Rik
UniProt Protein Name
WD repeat-containing protein 82
Protein Family
WD repeat-containing protein
UniProt Gene Name
Wdr82
UniProt Synonym Gene Names
Cdw5
NCBI Protein Information
WD repeat-containing protein 82

UniProt Comments for WDR82

WDR82: a WD repeat-containing protein. A regulatory component of the histone H3K4 methylatransferase SET1 complex implicated in tethering this complex to transcriptional start sites of active genes. Facilitates histone H3K4 methylation via recruitment of the SETD1A or SETD1B to phospho-S5 in the C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Component of the SET1 complex, composed of at least the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA, ¿B, or -C. Associated with multiple protein complexes including an RNA polymerase II complex, MLL3/MLL4 complex and a chaperonin-containing TCP1 complex. Interacts with CUL4B. Interacts with RBBP5 and SETD1B. Interacts with SETD1A (via RRM domain). Interacts with POLR2B. Interacts with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). SETD1A enhances its interaction with POLR2A. Interacts with PPP1R10/PNUTS. Interacts with PPP1CA in the presence of PPP1R10/PNUTS.

Protein type: Methyltransferase

Chromosomal Location of Human Ortholog: 9|9 F1

Cellular Component: chromatin; histone methyltransferase complex; nuclear chromosome, telomeric region; nucleolus; nucleus

Molecular Function: chromatin binding; histone methyltransferase activity (H3-K4 specific)

Biological Process: histone H3-K4 methylation
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Research Articles on WDR82

1. the result of a blocked WDR82 consists of stunted embryonic development and death. Thus, WDR82 can be considered a key epigenetic regulation-related factor crucial in the normal growth and development of embryos.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with WDR82 elisa kit

 Products by Pathway  Pathway Diagram
 MRNA Surveillance Pathway antibodies  MRNA Surveillance Pathway Diagram
 MRNA Surveillance Pathway antibodies  MRNA Surveillance Pathway Diagram

Diseases associated with WDR82 elisa kit

 Disease Name  Pubmed Publications
 Leukemia, Myeloid Antibodies  >1 publications with WDR82 and Leukemia, Myeloid
 Leukemia Antibodies  >1 publications with WDR82 and Leukemia

Organs/Tissues associated with WDR82 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >1 publications with WDR82 and Muscle
 Fertilized Ovum Antibodies  >1 publications with WDR82 and Fertilized Ovum
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