Zinc Finger MYM-Type Protein 2 (ZMYM2) ELISA Kit for Mouse

These translocations were associated with aberrant transcripts in which 4 predicted zinc finger domains, contributed by the widely expressed chromosome 13 gene ZNF198, were fused to the FGFR1 tyrosine-kinase domain. Transient expression studies showed that the ZNF198-FGFR1 fusion transcript directed the synthesis of an approximately 87-kD polypeptide, localizing predominantly to the cytoplasm.
The novel sequence that was fused to FGFR1, comprising the 5-prime end of the fusion transcript, was mapped to chromosome 13 with the CEPH mega-YAC library and the Genebridge 4 radiation hybrid panel. The findings were consistent with the pericentromeric 13q11-q12 assignments for the t(8;13) cytogenetic breakpoints.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZMYM2 in samples. An antibody specific for ZMYM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZMYM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZMYM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZMYM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of ZMYM2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS282809 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Zinc finger MYM-type protein 2 (ZMYM2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ZMYM2. The ELISA analytical biochemical technique of the MBS282809 kit is based on ZMYM2 antibody-ZMYM2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ZMYM2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ZMYM2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).