Putative Pre-Mrna-Splicing Factor ATP-Dependent RNA Helicase DHX16 (DHX16) ELISA Kit for Human

Name: Human DHX16 ELISA Kit; MyBioSource
Brand: MyBioSource
SKU: MBS282882
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282882

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DHX16 in samples. An antibody specific for DHX16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDHX16 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16, ELISA Kit

Full Product Name

Human Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (DHX16) ELISA Kit

Product Gene Name

DHX16 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human DHX16. No significant cross-reactivity or interference between Human DHX16 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

119,264 Da

OMIM

AB001601 mRNA

3D Structure

ModBase 3D Structure for O60231

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of DHX16 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DHX16 purchase

MBS282882 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DHX16. The ELISA analytical biochemical technique of the MBS282882 kit is based on DHX16 antibody-DHX16 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DHX16 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DHX16. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DHX16. It may not necessarily be applicable to this product.

NCBI GI #

256000749

NCBI GeneID

8449

NCBI Accession #

NP_001157711.1

UniProt Primary Accession #

O60231

UniProt Secondary Accession #

O60322; Q5JP45; Q969X7; Q96QC1

UniProt Related Accession #

O60231

GenBank Nucleotide #

NM_001164239.1

NCBI Official Full Name

putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 isoform 2

NCBI Official Synonym Full Names

DEAH-box helicase 16

NCBI Official Symbol

DHX16

NCBI Official Synonym Symbols

DBP2; PRP8; Prp2; DDX16; PRPF2; PRO2014

UniProt Protein Name

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16

UniProt Synonym Protein Names

ATP-dependent RNA helicase #3; DEAH-box protein 16

Protein Family

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase

UniProt Gene Name

DHX16

UniProt Synonym Gene Names

DBP2; DDX16; KIAA0577

NCBI Protein Information

putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16

NCBI Summary for DHX16

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a functional homolog of fission yeast Prp8 protein involved in cell cycle progression. This gene is mapped to the MHC region on chromosome 6p21.3, a region where many malignant, genetic and autoimmune disease genes are linked. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

UniProt Comments for DHX16

DDX16: a DEAD box protein, which is a functional homolog of fission yeast Prp8 protein involved in cell cycle progression. Probable ATP-binding RNA helicase involved in pre-mRNA splicing.

Protein type: EC 3.6.4.13; Helicase; RNA processing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: cytoplasm; nucleoplasm; nucleus; spliceosomal complex

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; ATPase activity; protein binding; RNA binding; RNA helicase activity

Biological Process: mRNA splicing, via spliceosome; RNA splicing

Research Articles on DHX16

1. Data show that G patch domain and KOW motifs protein (GPKOW )interacted directly with the DEAH-box protein 16 (DHX16/hPRP2) and with RNA.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DHX16 elisa kit

Reference Product	PubMed Publications
GPKOW elisa kit	>2 publications with DHX16 and GPKOW
DHX38 elisa kit	>1 publications with DHX16 and DHX38

Pathways associated with DHX16 elisa kit

Products by Pathway	Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
Spliceosome Pathway antibodies	Spliceosome Pathway Diagram
MRNA Processing Pathway antibodies	MRNA Processing Pathway Diagram

Organs/Tissues associated with DHX16 elisa kit

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>1 publications with DHX16 and Embryonic Tissue