Poliovirus Receptor-Related Protein 4 (PVRL4) ELISA Kit for Human

Name: Human PVRL4 ELISA Kit; Poliovirus Receptor-Related Protein 4; MyBioSource
Brand: MyBioSource
SKU: MBS282903
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282903

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PVRL4 in samples. An antibody specific for PVRL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPVRL4 present is bound by the… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Poliovirus receptor-related protein 4 (PVRL4), ELISA Kit

Full Product Name

Human Poliovirus receptor-related protein 4 (PVRL4) ELISA Kit

Product Gene Name

PVRL4 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PVRL4. No significant cross-reactivity or interference between Human PVRL4 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

24,341 Da

OMIM

AF426163 mRNA

3D Structure

ModBase 3D Structure for Q96NY8

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PVRL4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PVRL4 purchase

MBS282903 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Poliovirus receptor-related protein 4 (PVRL4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PVRL4. The ELISA analytical biochemical technique of the MBS282903 kit is based on PVRL4 antibody-PVRL4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PVRL4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PVRL4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PVRL4. It may not necessarily be applicable to this product.

NCBI GI #

222136611

NCBI GeneID

81607

NCBI Accession #

NP_112178.2

UniProt Primary Accession #

Q96NY8

UniProt Secondary Accession #

Q96K15; B4DQW3

UniProt Related Accession #

Q96NY8

GenBank Nucleotide #

NM_030916.2

NCBI Official Full Name

nectin-4

NCBI Official Synonym Full Names

nectin cell adhesion molecule 4

NCBI Official Symbol

NECTIN4

NCBI Official Synonym Symbols

LNIR; PRR4; EDSS1; PVRL4; nectin-4

UniProt Protein Name

Nectin-4

UniProt Synonym Protein Names

Ig superfamily receptor LNIR; Nectin cell adhesion molecule 4

Protein Family

Nectin

UniProt Gene Name

NECTIN4

NCBI Protein Information

nectin-4

NCBI Summary for PVRL4

This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011]

UniProt Comments for PVRL4

Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.

Research Articles on PVRL4

1. Nectin-4 is not only a BCSC marker but also a breast cancer metastasis marker

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PVRL4 elisa kit

Reference Product	PubMed Publications
PVRL1 elisa kit	>5 publications with PVRL4 and PVRL1
MLLT4 elisa kit	>1 publications with PVRL4 and MLLT4

Pathways associated with PVRL4 elisa kit

Products by Pathway	Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junctions Interactions Pathway antibodies	Adherens Junctions Interactions Pathway Diagram
Cell Junction Organization Pathway antibodies	Cell Junction Organization Pathway Diagram
Cell-Cell Communication Pathway antibodies	Cell-Cell Communication Pathway Diagram
Cell-cell Junction Organization Pathway antibodies	Cell-cell Junction Organization Pathway Diagram
Nectin/Necl Trans Heterodimerization Pathway antibodies	Nectin/Necl Trans Heterodimerization Pathway Diagram

Diseases associated with PVRL4 elisa kit

Disease Name	Pubmed Publications
ECTODERMAL DYSPLASIA-SYNDACTYLY SYNDROME 1 Antibodies	>4 publications with PVRL4 and ECTODERMAL DYSPLASIA-SYNDACTYLY SYNDROME 1
Neoplasms Antibodies	>2 publications with PVRL4 and Neoplasms
Fever Antibodies	>1 publications with PVRL4 and Fever
Necrosis Antibodies	>1 publications with PVRL4 and Necrosis