Human SMARCA2 ELISA Kit – Cat# MBS282943

Probable Global Transcription Activator SNF2L2 (SMARCA2) ELISA Kit for Human

Name: Human SMARCA2 ELISA Kit; Probable Global Transcription Activator SNF2L2; MyBioSource
Brand: MyBioSource
SKU: MBS282943
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282943

Intended Uses: For the quantitative detection of Human Probable global transcription activator SNF2L2 (SMARCA2) concentration in serum, plasma and other biological fluids.Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Probable global transcription activator SNF2L2 (SMARCA2), ELISA Kit

Full Product Name

Human Probable global transcription activator SNF2L2 (SMARCA2) ELISA Kit

Product Gene Name

SMARCA2 elisa kit

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Serum, plasma and other biological fluids

Detection Range

0.156 ng/mL - 10 ng/mL

Sensitivity

0.052 ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Human SMARCA2. No significant cross-reactivity or interference between Human SMARCA2 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%

Molecular Weight

179,281 Da

OMIM

X72889 mRNA

3D Structure

ModBase 3D Structure for P51531

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of SMARCA2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SMARCA2 purchase

MBS282943 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable global transcription activator SNF2L2 (SMARCA2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SMARCA2. The ELISA analytical biochemical technique of the MBS282943 kit is based on SMARCA2 antibody-SMARCA2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SMARCA2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SMARCA2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for SMARCA2. It may not necessarily be applicable to this product.

NCBI GI #

574957243

NCBI GeneID

6595

NCBI Accession #

NP_001276325.1

UniProt Primary Accession #

P51531

UniProt Secondary Accession #

B1ALG3; B1ALG4; D3DRH4; D3DRH5

UniProt Related Accession #

P51531

GenBank Nucleotide #

NM_001289396.1

NCBI Official Full Name

probable global transcription activator SNF2L2 isoform a

NCBI Official Synonym Full Names

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2

NCBI Official Symbol

SMARCA2

NCBI Official Synonym Symbols

BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a

UniProt Protein Name

Probable global transcription activator SNF2L2

UniProt Synonym Protein Names

ATP-dependent helicase SMARCA2; BRG1-associated factor 190B; BAF190B; Protein brahma homolog; hBRM; SNF2-alpha; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2

Protein Family

Probable global transcription activator

UniProt Gene Name

SMARCA2

UniProt Synonym Gene Names

BAF190B; hBRM

NCBI Protein Information

probable global transcription activator SNF2L2

NCBI Summary for SMARCA2

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism. [provided by RefSeq, Jan 2014]

UniProt Comments for SMARCA2

SMARCA2: an enzyme with ATP-dependent DNA helicase activity. A transcriptional coactivator that cooperates with nuclear hormone receptors to potentiate transcriptional activation. Binds TOPBP1. Belongs to the SNF2/RAD54 helicase family. Two alternatively spliced human isoforms have been described.

Protein type: EC 3.6.1.-; EC 3.6.4.-; Helicase; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9p24.3

Cellular Component: intermediate filament cytoskeleton; intracellular membrane-bound organelle; nBAF complex; npBAF complex; nuclear chromatin; nucleoplasm; nucleus; SWI/SNF complex

Molecular Function: ATP binding; chromatin binding; DNA-dependent ATPase activity; helicase activity; histone binding; protein binding; transcription coactivator activity; transcription regulatory region DNA binding

Biological Process: chromatin remodeling; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nervous system development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; spermatid development; transcription from RNA polymerase II promoter

Disease: Nicolaides-baraitser Syndrome

Research Articles on SMARCA2

1. Two BRM promoter polymorphisms were strongly associated with hepatocellular carcinoma (HCC) prognosis but were not associated with increased HCC susceptibility.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SMARCA2 elisa kit

Reference Product	PubMed Publications
SMARCA4 elisa kit	>195 publications with SMARCA2 and SMARCA4
SMARCB1 elisa kit	>22 publications with SMARCA2 and SMARCB1
ARID1A elisa kit	>17 publications with SMARCA2 and ARID1A
SMARCE1 elisa kit	>11 publications with SMARCA2 and SMARCE1
SMARCC1 elisa kit	>4 publications with SMARCA2 and SMARCC1
SS18 elisa kit	>3 publications with SMARCA2 and SS18
SMARCC2 elisa kit	>3 publications with SMARCA2 and SMARCC2
ACTL6A elisa kit	>2 publications with SMARCA2 and ACTL6A
SMARCD1 elisa kit	>1 publications with SMARCA2 and SMARCD1
CEBPB elisa kit	>1 publications with SMARCA2 and CEBPB

Pathways associated with SMARCA2 elisa kit

Products by Pathway	Pathway Diagram
C-MYB Transcription Factor Network Pathway antibodies	C-MYB Transcription Factor Network Pathway Diagram
Chromatin Modifying Enzymes Pathway antibodies	Chromatin Modifying Enzymes Pathway Diagram
Chromatin Organization Pathway antibodies	Chromatin Organization Pathway Diagram
E2F Transcription Factor Network Pathway antibodies	E2F Transcription Factor Network Pathway Diagram
RB In Cancer Pathway antibodies	RB In Cancer Pathway Diagram
RMTs Methylate Histone Arginines Pathway antibodies	RMTs Methylate Histone Arginines Pathway Diagram
Regulation Of Androgen Receptor Activity Pathway antibodies	Regulation Of Androgen Receptor Activity Pathway Diagram

Diseases associated with SMARCA2 elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>14 publications with SMARCA2 and Nervous System Diseases
Congenital Abnormalities Antibodies	>10 publications with SMARCA2 and Congenital Abnormalities
Nicolaides Baraitser syndrome Antibodies	>9 publications with SMARCA2 and Nicolaides Baraitser syndrome
Liver Neoplasms Antibodies	>8 publications with SMARCA2 and Liver Neoplasms
Liver Diseases Antibodies	>7 publications with SMARCA2 and Liver Diseases
Coffin-Siris syndrome Antibodies	>7 publications with SMARCA2 and Coffin-Siris syndrome
Inflammation Antibodies	>6 publications with SMARCA2 and Inflammation
Abnormalities, Multiple Antibodies	>6 publications with SMARCA2 and Abnormalities, Multiple
Disease Models, Animal Antibodies	>6 publications with SMARCA2 and Disease Models, Animal
Carcinoma, Hepatocellular Antibodies	>5 publications with SMARCA2 and Carcinoma, Hepatocellular

Organs/Tissues associated with SMARCA2 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>19 publications with SMARCA2 and Brain
Lung Antibodies	>18 publications with SMARCA2 and Lung
Liver Antibodies	>16 publications with SMARCA2 and Liver
Blood Antibodies	>13 publications with SMARCA2 and Blood
Embryonic Tissue Antibodies	>11 publications with SMARCA2 and Embryonic Tissue
Testis Antibodies	>9 publications with SMARCA2 and Testis
Heart Antibodies	>8 publications with SMARCA2 and Heart
Vascular Antibodies	>7 publications with SMARCA2 and Vascular
Skin Antibodies	>6 publications with SMARCA2 and Skin
Prostate Antibodies	>6 publications with SMARCA2 and Prostate