Probable Lysosomal Cobalamin Transporter (LMBRD1) ELISA Kit for Human

Name: Human LMBRD1 ELISA Kit; Probable Lysosomal Cobalamin Transporter; MyBioSource
Brand: MyBioSource
SKU: MBS282967
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS282967

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate LMBRD1 in samples. An antibody specific for LMBRD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLMBRD1 present is bound by… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Probable lysosomal cobalamin transporter (LMBRD1), ELISA Kit

Full Product Name

Human Probable lysosomal cobalamin transporter (LMBRD1) ELISA Kit

Product Gene Name

LMBRD1 elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human LMBRD1. No significant cross-reactivity or interference between Human LMBRD1 and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

21,394 Da

OMIM

AY136817 mRNA

3D Structure

ModBase 3D Structure for Q9NUN5

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of LMBRD1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LMBRD1 purchase

MBS282967 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Probable lysosomal cobalamin transporter (LMBRD1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LMBRD1. The ELISA analytical biochemical technique of the MBS282967 kit is based on LMBRD1 antibody-LMBRD1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LMBRD1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LMBRD1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LMBRD1. It may not necessarily be applicable to this product.

NCBI GI #

261878497

NCBI GeneID

55788

NCBI Accession #

NP_060838.3

UniProt Primary Accession #

Q9NUN5

UniProt Secondary Accession #

Q5VUN6; Q86Y70; Q96FW4; Q9BY56; Q9NZD6; A8K204; E1P531

UniProt Related Accession #

Q9NUN5

GenBank Nucleotide #

NM_018368.3

NCBI Official Full Name

probable lysosomal cobalamin transporter

NCBI Official Synonym Full Names

LMBR1 domain containing 1

NCBI Official Symbol

LMBRD1

NCBI Official Synonym Symbols

NESI; LMBD1; MAHCF; C6orf209

UniProt Protein Name

Probable lysosomal cobalamin transporter

UniProt Synonym Protein Names

HDAg-L-interacting protein NESI; LMBR1 domain-containing protein 1; Nuclear export signal-interacting protein

Protein Family

Probable lysosomal cobalamin transporter

UniProt Gene Name

LMBRD1

UniProt Synonym Gene Names

C6orf209; NESI

NCBI Protein Information

probable lysosomal cobalamin transporter

NCBI Summary for LMBRD1

This gene encodes a lysosomal membrane protein that may be involved in the transport and metabolism of cobalamin. This protein also interacts with the large form of the hepatitis delta antigen and may be required for the nucleocytoplasmic shuttling of the hepatitis delta virus. Mutations in this gene are associated with the vitamin B12 metabolism disorder termed, homocystinuria-megaloblastic anemia complementation type F.[provided by RefSeq, Oct 2009]

UniProt Comments for LMBRD1

LMBRD1: Probable lysosomal cobalamin transporter. Required to export cobalamin from lysosomes allowing its conversion to cofactors. Isoform 3 may play a role in the assembly of hepatitis delta virus (HDV). Defects in LMBRD1 are the cause of methylmalonic aciduria and homocystinuria type cblF (MMAHCF). A disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). It is due to accumulation of free cobalamin in lysosomes, thus hindering its conversion to cofactors. Clinical features include developmental delay, stomatitis, glossitis, seizures and methylmalonic aciduria responsive to vitamin B12. Belongs to the LIMR family. LMBRD1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 6q13

Cellular Component: clathrin-coated endocytic vesicle; integral component of membrane; lysosomal membrane; membrane; plasma membrane

Molecular Function: cobalamin binding; cobalamin transporter activity; insulin receptor binding

Biological Process: cobalamin metabolic process; cobalamin transport; negative regulation of glucose import; negative regulation of insulin receptor signaling pathway; negative regulation of protein kinase B signaling cascade; viral process

Disease: Methylmalonic Aciduria And Homocystinuria, Cblf Type

Research Articles on LMBRD1

1. Data suggest that ABCD4 lysosomal targeting depends on co-expression of and interaction with LMBRD1; mutations in LMBRD1 and ABCD4 that result in cobalamin metabolism disorders cblF and cblJ (or mutations in ATPase domain) disrupt interactions between LMBRD1 and ABCD4. (LMBRD1 = nuclear export signal-interacting protein; ABCD4 = ATP-binding cassette, sub-family D (ALD), member 4)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LMBRD1 elisa kit

Reference Product	PubMed Publications
MTR elisa kit	>2 publications with LMBRD1 and MTR
MMADHC elisa kit	>1 publications with LMBRD1 and MMADHC

Pathways associated with LMBRD1 elisa kit

Products by Pathway	Pathway Diagram
Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway antibodies	Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway Diagram
Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies	Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
Defective BTD Causes Biotidinase Deficiency Pathway antibodies	Defective BTD Causes Biotidinase Deficiency Pathway Diagram
Defective CD320 Causes Methylmalonic Aciduria Pathway antibodies	Defective CD320 Causes Methylmalonic Aciduria Pathway Diagram
Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway antibodies	Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway Diagram
Defective GIF Causes Intrinsic Factor Deficiency Pathway antibodies	Defective GIF Causes Intrinsic Factor Deficiency Pathway Diagram
Defective HLCS Causes Multiple Carboxylase Deficiency Pathway antibodies	Defective HLCS Causes Multiple Carboxylase Deficiency Pathway Diagram
Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway antibodies	Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway Diagram
Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway antibodies	Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway Diagram
Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway antibodies	Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway Diagram

Diseases associated with LMBRD1 elisa kit

Disease Name	Pubmed Publications
Anemia, Hemolytic Antibodies	>1 publications with LMBRD1 and Anemia, Hemolytic
Neurobehavioral Manifestations Antibodies	>1 publications with LMBRD1 and Neurobehavioral Manifestations
Methylmalonic Aciduria and Homocystinuria, CblF Type Antibodies	>1 publications with LMBRD1 and Methylmalonic Aciduria and Homocystinuria, CblF Type

Organs/Tissues associated with LMBRD1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>5 publications with LMBRD1 and Blood
Heart Antibodies	>4 publications with LMBRD1 and Heart
Skin Antibodies	>3 publications with LMBRD1 and Skin
Nerve Antibodies	>1 publications with LMBRD1 and Nerve
Liver Antibodies	>1 publications with LMBRD1 and Liver
Muscle Antibodies	>1 publications with LMBRD1 and Muscle