Plexin-A3 (PLXNA3) ELISA Kit for Human

SEX shares significant homology with the extracellular domain of the receptors encoded by the oncogenes MET ,RON, and SEA. The proteins encoded by these genes contain large cytoplasmic domains characterized by a distinctive highly conserved sequence, which they called the SEX domain. Northern blot analysis revealed different expression of the SEX family of genes in fetal tissues, with SEX, OCT, and NOV predominantly expressed in brain, and SEP expressed at highest levels in kidney. In situ hybridization analysis revealed that a mouse SEX homolog has a distinctive pattern of expression in the developing nervous system of the mouse, where it is found in postmitotic neurons from the first stages of neuronal differentiation. The SEX protein (220 kD) is glycosylated and exposed at the cell surface.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PLXNA3 in samples. An antibody specific for PLXNA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLXNA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLXNA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLXNA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of PLXNA3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS282968 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Plexin-A3 (PLXNA3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PLXNA3. The ELISA analytical biochemical technique of the MBS282968 kit is based on PLXNA3 antibody-PLXNA3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PLXNA3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PLXNA3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).