Dual Specificity Tyrosine-Phosphorylation-Regulated Kinase 1a (DYRK1A) ELISA Kit for Rabbit

DYRK1A encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DYRK1A in samples. An antibody specific for DYRK1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDYRK1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DYRK1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DYRK1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of DYRK1A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283002 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DYRK1A. The ELISA analytical biochemical technique of the MBS283002 kit is based on DYRK1A antibody-DYRK1A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DYRK1A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DYRK1A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).