Methylmalonyl-Coa Epimerase (MCEE) ELISA Kit for Mouse

Methylmalonyl CoA Epimerase catalyzes the interconversion of D- and L-methylmalonyl-CoA during the degradation of branched chain amino acids. odd chain-length fatty acids, and other metabolites. Mutations in this gene result in methylmalonyl-CoA epimerase deficiency, which is presented as mild to moderate methylmalonic aciduria.Bikker et al. (2006) reported a 16-year-old female patient with persistent moderate methylmalonic aciduria. In vitro biochemical findings that comprised a decreased propionate incorporation into macromolecules in cultured fibroblasts and a fully normal activity of methylmalonyl-CoA mutase in the same cells were fully explained by deficiency of methylmalonyl-CoA epimerase.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MCEE in samples. An antibody specific for MCEE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMCEE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCEE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCEE bound in the initial step. The color development is stopped and the intensity of the color is measured.

Small volumes of MCEE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS283022 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Methylmalonyl-CoA epimerase (MCEE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MCEE. The ELISA analytical biochemical technique of the MBS283022 kit is based on MCEE antibody-MCEE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MCEE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MCEE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).